Department of Clinical Microbiology, Biomedical Diagnostic Centre (CDB), Hospital Clinic, School of Medicine, University of Barcelona, Villarroel, 170, 08036 Barcelona, Spain.
ISGlobal, Barcelona Centre for International Health Research (CRESIB), Barcelona, Spain.
J Travel Med. 2017 Apr 1;24(suppl_1):S23-S28. doi: 10.1093/jtm/taw071.
Traveller's diarrhea can be caused by bacteria, protozoa, helminths and viruses. Globally, the most common causes of traveller's diarrhea are two pathotypes of Escherichia coli (enterotoxigenic and enteroaggregative) and Shigella, although there are significant variations according to the geographic area visited. While traveller's diarrhea is usually a mild, self-limiting disease, half of the travellers with traveller's diarrhea have some limitation in their activities during the journey and up to 10% present persistent diarrhea or other complications, making microbiological diagnosis necessary. The aim of this article is to describe the application of new molecular diagnostic tools mainly based on multiplex PCR, including their advantages and disadvantages as well as the current gaps that requiring further study.
旅行者腹泻可由细菌、原生动物、蠕虫和病毒引起。在全球范围内，旅行者腹泻最常见的病因是两种产肠毒素和聚集性大肠杆菌（enterotoxigenic 和 enteroaggregative）以及志贺氏菌，尽管根据所访问的地理区域存在显著差异。虽然旅行者腹泻通常是一种轻度、自限性疾病，但一半的旅行者腹泻患者在旅行期间其活动受到一些限制，高达 10%的患者出现持续性腹泻或其他并发症，这使得微生物学诊断成为必要。本文的目的是描述主要基于多重 PCR 的新型分子诊断工具的应用，包括它们的优缺点以及目前需要进一步研究的差距。
