Intracellular recordings from renin-positive cells of the afferent glomerular arteriole.

Intracellular recordings were made in juxtaglomerular granulated (JG) cells and in vascular smooth muscle (VSM) cells in afferent arterioles of hydronephrotic mouse kidneys. Both cell types did not differ in their passive and active electrical membrane properties; membrane potential was about -58 mV, input resistance exceeded 400 M omega, and JG as well as VSM cells showed spontaneous depolarizations resembling excitatory junction potentials and active responses observed in smooth muscle cells of other blood vessels in various species. These depolarizations, attributed to spontaneous transmitter release from adrenergic terminals, were extremely polymorphous and quite frequent. Epinephrine, norepinephrine, phenylephrine, arginine vasopressin, and angiotensin II depolarized JG and VSM cells, but isoproterenol and orciprenaline had no effect. A hyperpolarizing action of catecholamines was never observed. It is suggested that, in this in vitro preparation, isoproterenol increases renin secretion by a mechanism independent of membrane potential changes. Depolarizations mediated by alpha-mimetic agents, arginine vasopressin, and angiotensin II, as well as by the junctional activity may inhibit renin secretion by an increased calcium influx into JG cells.