Kolmogorova Daria, Murray Emma, Ismail Nafissa
School of Psychology, Faculty of Social Sciences, University of Ottawa, Ottawa, Ontario, Canada.
Brain and Mind Research Institute, University of Ottawa, Ottawa, Ontario, Canada.
Curr Protoc Mouse Biol. 2017 Jun 19;7(2):65-76. doi: 10.1002/cpmo.27.
Sickness behavior monitoring, a technique for examining the development of sickness symptomatology following infection, is necessary in experiments studying neurochemical and physiological changes associated with pathogen-induced immune activation. However, the results of sickness behavior monitoring are difficult to reconcile due to inconsistencies in protocol methods and rater bias. The protocol described herein offers a non-invasive and unbiased approach to assess the progression of pathogen-induced sickness behaviors. This simple, straightforward method uses a five-point scale to assess animals for the presence of four sickness behaviors (i.e., '"0" = no sickness behaviors; "4" = four sickness behaviors) at various time points following exposure to a pathogen. This approach removes the ambiguity and bias inherent to other methods of sickness behavior monitoring that rely on subjective ratings of severity for individual symptoms. This protocol has been successfully applied to male and female rodents injected intraperitoneally with lipopolysaccharide and polyinosinic:polycytidylic acid, and has been effective in pubertal and adult populations. Protocols for changes in body temperature and weight are also provided as physiological markers of sickness. © 2017 by John Wiley & Sons, Inc.
疾病行为监测是一种用于检查感染后疾病症状发展的技术，在研究与病原体诱导的免疫激活相关的神经化学和生理变化的实验中是必要的。然而，由于方案方法的不一致和评分者偏差，疾病行为监测的结果难以协调。本文所述的方案提供了一种非侵入性且无偏差的方法来评估病原体诱导的疾病行为的进展。这种简单直接的方法使用五点量表在动物接触病原体后的不同时间点评估其是否存在四种疾病行为（即，“0”=无疾病行为；“4”=四种疾病行为）。这种方法消除了其他疾病行为监测方法所固有的模糊性和偏差，其他方法依赖于对个体症状严重程度的主观评分。该方案已成功应用于腹腔注射脂多糖和聚肌苷酸:聚胞苷酸的雄性和雌性啮齿动物，并在青春期和成年群体中有效。还提供了体温和体重变化的方案作为疾病的生理指标。© 2017约翰威立国际出版公司。
