Nakano M, Sugioka K, Nakano H, Takyu C, Inaba H
Biochem Biophys Res Commun. 1985 Aug 15;130(3):952-6. doi: 10.1016/0006-291x(85)91707-3.
Chemiluminescence in visible region was detected, during peroxidase-catalyzed oxidation of chlorpromazine at pH7.5 (but not at pH4.25) or of propericiazine, both at pH7.5 and at pH4.25. Red colored intermediates, cation radicals, were produced and decayed in all enzymatic systems used. Chemiluminescence was also detected at pH7.5, but not at pH4.25, when synthesized cation radical of chlorpromazine in water was mixed with concentrated buffer. Protonation of alkyl nitrogen in the cation radical of chlorpromazine was highly related to the appearance of luminescence. Possible mechanisms of the generation of the excited phenothiazine analogs were discussed.
在pH7.5（而非pH4.25）条件下，过氧化物酶催化氯丙嗪氧化时，或者在pH7.5和pH4.25条件下，过氧化物酶催化丙酰奋乃静氧化时，均可检测到可见区域的化学发光现象。在所使用的所有酶促体系中，均会产生并衰减红色中间体——阳离子自由基。当水中合成的氯丙嗪阳离子自由基与浓缩缓冲液混合时，在pH7.5条件下也可检测到化学发光现象，但在pH4.25条件下则未检测到。氯丙嗪阳离子自由基中烷基氮的质子化与发光现象的出现高度相关。文中讨论了激发态吩噻嗪类似物产生的可能机制。
