Rubio Camila Peres, Yilmaz Zeki, Martínez-Subiela Silvia, Kocaturk Meric, Hernández-Ruiz Josefa, Yalcin Ebru, Tvarijonaviciute Asta, Escribano Damián, Ceron Jose Joaquín
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100, Espinardo, Murcia, Spain.
Department of Internal Medicine, Faculty of Veterinary Medicine, Uludag University, 16059 Bursa, Turkey.
Res Vet Sci. 2017 Dec;115:301-306. doi: 10.1016/j.rvsc.2017.06.004. Epub 2017 Jun 12.
The objective of this study was to evaluate and compare the antioxidant response and the products of oxidative damage analysed by various assays in clinical and subclinical canine monocytic ehrlichiosis (CME). For this purpose, four assays to measure the total serum antioxidant capacity (TAC), such as the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC) using acidic medium (TEAC), and the TEAC using the horseradish peroxidase (TEAC) were used. In addition, the serum thiol concentrations were analysed. Reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and ferrous oxidation-xylenol orange (FOX) were measured to determine the concentrations of free radical and the products of oxidative damage as result of the disease. All antioxidant markers were significantly lower in the dogs on clinical ehrlichiosis when compared with healthy dogs; however only the CUPRAC, FRAP and thiol were significantly lower in subclinical CME compared with healthy dogs. TBARS and FOX showed no significant differences between dogs with CME and healthy dogs; however, a significant increased ROS concentration was observed in dogs with clinical and subclinical CME when compared with healthy dogs. Results showed that in CME there is a state of oxidative stress with significant changes in markers of antioxidant defence and in concentrations of free radicals. However, the detection of these changes would depend of the assay used.
本研究的目的是评估和比较通过各种检测方法分析的临床和亚临床犬单核细胞埃立克体病（CME）中的抗氧化反应和氧化损伤产物。为此，使用了四种检测血清总抗氧化能力（TAC）的方法，如铜离子还原抗氧化能力（CUPRAC）、血浆铁还原能力（FRAP）、使用酸性介质的Trolox等效抗氧化能力（TEAC）以及使用辣根过氧化物酶的TEAC。此外，还分析了血清硫醇浓度。测量了活性氧（ROS）、硫代巴比妥酸反应性物质（TBARS）和亚铁氧化二甲苯酚橙（FOX），以确定疾病导致的自由基浓度和氧化损伤产物。与健康犬相比，临床埃立克体病犬的所有抗氧化标志物均显著降低；然而，与健康犬相比，亚临床CME中只有CUPRAC、FRAP和硫醇显著降低。TBARS和FOX在CME犬和健康犬之间没有显著差异；然而，与健康犬相比，临床和亚临床CME犬中观察到ROS浓度显著升高。结果表明，在CME中存在氧化应激状态，抗氧化防御标志物和自由基浓度有显著变化。然而，这些变化的检测将取决于所使用的检测方法。
