Mohammadian Farideh, Pilehvar-Soltanahmadi Younes, Alipour Shahriar, Dadashpour Mehdi, Zarghami Nosratollah
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Res (Stuttg). 2017 Sep;67(9):509-514. doi: 10.1055/s-0042-119647. Epub 2017 Jun 19.
Gastric carcinoma still remains the second most common cause of cancer mortality in the world. Chrysin, as a flavone, has showed cancer chemopreventive activity. The cellular and molecular mechanisms of chrysin in cancer cells have not been fully understood. In this study, we investigate expression levels of let-7a, miR-9, mir-18a, miR-21, miR-22, miR-34a, miR-126 and mir-221 to describe the anti-cancer effects of chrysin. The cytotoxic effects of chrysin were assessed using MTT assay. The effect of chrysin on the microRNAs expression was determined by qRT-PCR. The MTT results for different concentrations of chrysin at different times on the Gastric carcinoma cells showed that IC50 for chrysin was 68.24 µM after 24 h of treatment. Expression analysis identified that miR-18, miR-21 and miR-221 were down regulated whereas let-7a, miR-9, miR-22, miR-34a and miR-126 were up regulated in Gastric carcinoma cell line (p<0.05). Treatment with chrysin can alter the miRNAs expression and these findings might be an explanation for molecular mechanism of chrysin effect on gastric cancer.
胃癌仍然是全球癌症死亡的第二大常见原因。白杨素作为一种黄酮类化合物，已显示出癌症化学预防活性。白杨素在癌细胞中的细胞和分子机制尚未完全明确。在本研究中，我们检测了let-7a、miR-9、mir-18a、miR-21、miR-22、miR-34a、miR-126和mir-221的表达水平，以描述白杨素的抗癌作用。采用MTT法评估白杨素的细胞毒性作用。通过qRT-PCR测定白杨素对微小RNA表达的影响。不同浓度的白杨素在不同时间对胃癌细胞的MTT结果显示，处理24小时后白杨素的IC50为68.24µM。表达分析表明，在胃癌细胞系中，miR-18、miR-21和miR-221表达下调，而let-7a、miR-9、miR-22、miR-34a和miR-126表达上调（p<0.05）。白杨素处理可改变微小RNA的表达，这些发现可能解释了白杨素对胃癌作用的分子机制。
