Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:108-115. doi: 10.1016/j.msec.2017.05.006. Epub 2017 May 4.
Search for biologically active nanoparticles is prerequisite for biomedical applications. CuO nanoparticles synthesized by co-precipitation method are capped by polyethylene-glycol (PEG) and polyvinyl-pyrrolidone (PVP) on the surface by simple adsorption. Physical and chemical properties carried out by SEM, XRD and FTIR confirm nanometer in size and efficient capping of PVP and PEG on CuO NPs. Biological assays reveal higher activities of CuO-PEG and CuO-PVP as compared to the uncapped CuO nanoparticles. CuO-PEG shows better antitumor activity against Streptomyces as compared with CuO-PVP and CuO NPs. Both the capped NPs are significantly active for α-amylase inhibition assay. CuO-PVP demonstrates significantly better activity against bacterial strains followed by CuO-PEG and uncapped CuO. PVP coated CuO NPs also shows strong DPPH based free radical scavenging activity, total reducing power potential, total antioxidative potential and also carries flavonoid and phenolics properties determines to querecetin and gallic acid equivalence, respectively. It can be concluded that PVP and PEG capped CuO NPs are more capable to be used in biomedical applications as drug and diagnostic carrier molecules.
寻找具有生物活性的纳米粒子是生物医学应用的前提。通过共沉淀法合成的氧化铜纳米粒子通过简单吸附在表面上被聚乙二醇（PEG）和聚乙烯吡咯烷酮（PVP）覆盖。通过 SEM、XRD 和 FTIR 进行的物理和化学性质证实了纳米尺寸和 PVP 和 PEG 对 CuO NPs 的有效覆盖。生物测定表明，与未覆盖的 CuO 纳米粒子相比，CuO-PEG 和 CuO-PVP 具有更高的活性。与 CuO-PVP 和 CuO NPs 相比，CuO-PEG 对链霉菌具有更好的抗肿瘤活性。两种覆盖的纳米粒子在α-淀粉酶抑制测定中均具有显著的活性。CuO-PVP 对细菌菌株的活性明显优于 CuO-PEG 和未覆盖的 CuO。PVP 包覆的 CuO NPs 还表现出很强的 DPPH 自由基清除活性、总还原能力潜力、总抗氧化潜力，并具有类黄酮和酚类特性，分别确定为槲皮素和没食子酸当量。可以得出结论，PVP 和 PEG 包覆的 CuO NPs 更有能力作为药物和诊断载体分子应用于生物医学领域。
