Faculty of Industrial Sciences & Technology, University Malaysia Pahang, Lebuhraya Tun Razak, Gambang, 26300 Kuantan, Pahang, Malaysia.
Faculty of Industrial Sciences & Technology, University Malaysia Pahang, Lebuhraya Tun Razak, Gambang, 26300 Kuantan, Pahang, Malaysia.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:151-160. doi: 10.1016/j.msec.2017.05.028. Epub 2017 May 8.
Green porous and ecofriendly scaffolds have been considered as one of the potent candidates for tissue engineering substitutes. The objective of this study is to investigate the biocompatibility of hydroxyethyl cellulose (HEC)/silver nanoparticles (AgNPs), prepared by the green synthesis method as a potential host material for skin tissue applications. The substrates which contained varied concentrations of AgNO (0.4%-1.6%) were formed in the presence of HEC, were dissolved in a single step in water. The presence of AgNPs was confirmed visually by the change of color from colorless to dark brown, and was fabricated via freeze-drying technique. The outcomes exhibited significant porosity of >80%, moderate degradation rate, and tremendous value of water absorption up to 1163% in all samples. These scaffolds of HEC/AgNPs were further characterized by SEM, UV-Vis, ATR-FTIR, TGA, and DSC. All scaffolds possessed open interconnected pore size in the range of 50-150μm. The characteristic peaks of Ag in the UV-Vis spectra (417-421nm) revealed the formation of AgNPs in the blend composite. ATR-FTIR curve showed new existing peak, which implies the oxidation of HEC in the cellulose derivatives. The DSC thermogram showed augmentation in T with increased AgNO concentration. Preliminary studies of cytotoxicity were carried out in vitro by implementation of the hFB cells on the scaffolds. The results substantiated low toxicity of HEC/AgNPs scaffolds, thus exhibiting an ideal characteristic in skin tissue engineering applications.
绿色多孔且环保的支架被认为是组织工程替代物的有力候选物之一。本研究的目的是研究羟乙基纤维素（HEC）/银纳米颗粒（AgNPs）的生物相容性，这些颗粒是通过绿色合成方法制备的，可用作皮肤组织应用的潜在宿主材料。在 HEC 的存在下形成了含有不同浓度 AgNO（0.4%-1.6%）的基质，然后可在一步中溶解在水中。AgNPs 的存在通过颜色从无色变为深褐色的变化来肉眼确认，并通过冷冻干燥技术制造。结果表明，所有样品的孔隙率>80%、适中的降解率以及高达 1163%的高吸水性。进一步通过 SEM、UV-Vis、ATR-FTIR、TGA 和 DSC 对 HEC/AgNPs 支架进行了表征。所有支架都具有 50-150μm 范围内的开放互连的孔尺寸。在 UV-Vis 光谱（417-421nm）中的 Ag 的特征峰表明在共混复合材料中形成了 AgNPs。ATR-FTIR 曲线显示了新的存在峰，这意味着纤维素衍生物中 HEC 的氧化。DSC 热图谱显示随着 AgNO 浓度的增加 T 增加。通过将 hFB 细胞接种在支架上来进行体外细胞毒性的初步研究。结果证实了 HEC/AgNPs 支架的低毒性，因此在皮肤组织工程应用中表现出理想的特性。
