Xu Lian, Sun Ji-Quan, Wang Li-Juan, Gao Zhi-Wei, Sun Li-Zhu, Wu Xiao-Lei
Institute of Innovation (Baotou), Peking University, Baotou 014030, PR China.
College of Engineering, Peking University, Beijing 100871, PR China.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1943-1948. doi: 10.1099/ijsem.0.001895. Epub 2017 Jun 20.
A Gram-staining-negative, non-motile, non-spore-forming bacterium designated Y3L14T was isolated from the saline-alkaline soil of a farmland, Inner Mongolia, northern China. Strain Y3L14T could grow at 10-40 °C (optimally at 30 °C), pH 6.0-10.0 (optimally at pH 8.0), and in the presence of 0-6.0 % (w/v) NaCl (optimally with 0-2.0 %). Phylogenetic analysis based on the 16S rRNA gene and DNA gyrase subunit B (gyrB) gene sequences revealed that strain Y3L14T clustered with strains belonging to the genus Sphingobacterium, sharing the highest 16S rRNA gene sequence similarity with Sphingobacterium lactis WCC 4512T (94.99 %). Its major cellular fatty acids contained iso-C15 : 0, C16 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). Menaquinone-7 (MK-7) was the only isoprenoid quinone. Strain Y3L14T contained phosphatidylethanolamine, sphingophospholipid, two unknown phospholipids and three unknown lipids as the major polar lipids. The genomic DNA G+C content of strain Y3L14T was 36.0 mol%. Based on the phenotypic, phylogenetic and genotypic characteristics, strain Y3L14T represents a novel species within the genus Sphingobacterium, for which Sphingobacterium alkalisoli sp. nov. is proposed; the type strain is Y3L14T (=CGMCC 1.15782T=KCTC 52379T).
从中国北方内蒙古某农田盐碱土中分离出一株革兰氏染色阴性、无运动性、不产芽孢的细菌，命名为Y3L14T。菌株Y3L14T能在10-40 °C（最适温度30 °C）、pH 6.0-10.0（最适pH 8.0）以及0-6.0%（w/v）NaCl（最适0-2.0%）条件下生长。基于16S rRNA基因和DNA促旋酶亚基B（gyrB）基因序列的系统发育分析表明，菌株Y3L14T与鞘氨醇杆菌属的菌株聚类，与乳酸鞘氨醇杆菌WCC 4512T的16S rRNA基因序列相似性最高（94.99%）。其主要细胞脂肪酸包括异-C15 : 0、C16 : 0、异-C17 : 0 3-OH以及特征性总和3（异-C15 : 0 2-OH和/或C16 : 1ω7c）。甲基萘醌-7（MK-7）是唯一的类异戊二烯醌。菌株Y3L14T含有磷脂酰乙醇胺、鞘磷脂、两种未知磷脂和三种未知脂类作为主要极性脂类。菌株Y3L14T的基因组DNA G+C含量为36.0 mol%。基于表型、系统发育和基因型特征，菌株Y3L14T代表鞘氨醇杆菌属内的一个新物种，为此提出盐碱鞘氨醇杆菌新种；模式菌株为Y3L14T（=CGMCC 1.15782T=KCTC 52379T）。
