Gai Yingbao, Huang Zhaobin, Lai Qiliang, Shao Zongze
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, PR China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State of Oceanic Administration, Xiamen, PR China.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1901-1905. doi: 10.1099/ijsem.0.001878. Epub 2017 Jun 20.
A Gram-stain-negative, rod-shaped bacterium with a singular polar flagellum, designated as strain XMDDZSB0408T, was isolated from the intestine of adult abalone, Haliotis diversicolor. Activity of oxidase was positive and catalase activity was negative. It could grow at salinities from 3 to 6 % NaCl (w/v), and pH 7-9. It had the highest sequence similarity of <96.0 % with all previously established species for the complete 16S rRNA gene (1531 bp). The results of phylogenetic analysis indicated that the strain was affiliated to the genus Shewanella and closely related to Shewanella gaetbuli TF-27T and Shewanella japonica KMM 3299T (95.8 % sequence similarity), Shewanella electrodiphila MAR441T (95.6 %), Shewanella pacifica KMM 3597T (95.4 %), Shewanella donghaensis LT17T (95.3 %) and Shewanella olleyana ACEM 9T (94.7 %). The respiratory quinones were MK-7, Q-8, Q-7, MK-8, Q-7 (H4) and Q-6. The predominant fatty acids consisted of C16:0, summed feature 3 (comprised of C16 : 1ω7c/C16 : 1ω6c), C18 : 0, summed feature 8 (comprised of C18 : 1ω7c/C18 : 1ω6c), C12 : 0 and C14 : 0. The polar lipids were identified as phosphatidylethanolamine (PE), a glycolipid (GL), a phospholipid (PL) and one unidentified lipid (L). The DNA G+C content was 41.4 mol% calculated from the draft genome sequence. On the basis of its polyphasic taxonomic properties, strain XMDDZSB0408T represented a novel species, for which the name Shewanella intestini sp. nov. was proposed, with the type strain XMDDZSB0408T (=KCTC 52125T=MCCC 1A01895T).
从杂色鲍成体肠道中分离出一株革兰氏染色阴性、杆状、具单根极生鞭毛的细菌，命名为菌株XMDDZSB0408T。氧化酶活性为阳性，过氧化氢酶活性为阴性。该菌能在3%至6%（w/v）NaCl盐度及pH值7至9的条件下生长。其16S rRNA基因全长（1531 bp）与所有已确立的物种的序列相似性最高<96.0%。系统发育分析结果表明，该菌株隶属于希瓦氏菌属，与加埃特布利希瓦氏菌TF-27T、日本希瓦氏菌KMM 3299T（序列相似性95.8%）、嗜电希瓦氏菌MAR441T（95.6%）、太平洋希瓦氏菌KMM 3597T（95.4%）、东海希瓦氏菌LT17T（95.3%）和奥氏希瓦氏菌ACEM 9T（94.7%）亲缘关系密切。呼吸醌为MK-7、Q-8、Q-7、MK-8、Q-7（H4）和Q-6。主要脂肪酸包括C16:0、特征性总和3（由C16 : 1ω7c/C16 : 1ω6c组成）、C18 : 0、特征性总和8（由C18 : 1ω7c/C18 : 1ω6c组成）、C12 : 0和C14 : 0。极性脂被鉴定为磷脂酰乙醇胺（PE）、一种糖脂（GL）、一种磷脂（PL）和一种未鉴定的脂类（L）。根据草图基因组序列计算，DNA G+C含量为41.4 mol%。基于其多相分类学特性，菌株XMDDZSB0408T代表一个新物种，提议将其命名为肠道希瓦氏菌（Shewanella intestini sp. nov.），模式菌株为XMDDZSB0408T（=KCTC 52125T=MCCC 1A01895T）。
