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sp. nov.,从鲍鱼的肠道中分离得到。

sp. nov., isolated from the intestine of abalone, .

机构信息

School of Marine Sciences, Ningbo University, Ningbo, PR China.

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, PR China.

出版信息

Int J Syst Evol Microbiol. 2019 Oct;69(10):3202-3206. doi: 10.1099/ijsem.0.003610.

Abstract

A Gram-stain-negative, rod-shaped and facultative anaerobic strain named YE-B6 was isolated from the intestine of abalone, . The reactions of oxidase and catalase were both positive. Strain YE-B6 could grow at 10-37 °C (optimum, 28-33 °C), at pH 7-9 (optimum, pH 7) and in salinity of 0-8 % NaCl (w/v; optimum, 2 %). The 16S rRNA gene sequence of strain YE-B6 had maximum sequence similarities to H1304 (98.0 %), DQHS21 (97.4 %), JC131 (97.4 %) and CL-GR15 (97.3 %). Results of phylogenetic analysis showed that it was affiliated to the genus within the order in the . The respiratory quinone of strain YE-B6 was Q-10. The cellular fatty acids mainly comprised C 7 and summed feature 3 (Cω6 and/or Cω7). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified aminolipids, two unidentified polar lipids and one unidentified phospholipid. The G+C content of strain YE-B6 was 55.6 mol%. Based on its genotypic, physiological and biochemical characteristics, strain YE-B6 represented a novel species within the genus , for which the name sp. nov. is proposed. The type strain of the new species is YE-B6 (=MCCC 1A13131=KCTC 62716).

摘要

一株从鲍鱼肠道中分离得到的革兰氏阴性、杆状、兼性厌氧菌株,命名为 YE-B6。氧化酶和过氧化氢酶反应均为阳性。菌株 YE-B6 可以在 10-37°C(最适温度为 28-33°C)、pH7-9(最适 pH 为 7)和 0-8%NaCl(w/v;最适 2%NaCl)的条件下生长。菌株 YE-B6 的 16S rRNA 基因序列与 H1304(98.0%)、DQHS21(97.4%)、JC131(97.4%)和 CL-GR15(97.3%)的相似度最高。系统发育分析结果表明,该菌株属于 属,在 目 科中。菌株 YE-B6 的呼吸醌为 Q-10。细胞脂肪酸主要由 C 7 和特征性脂肪酸 3(Cω6 和/或 Cω7)组成。极性脂类主要由二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰单甲乙醇胺、两种未鉴定的胺脂、两种未鉴定的极性脂类和一种未鉴定的磷脂组成。菌株 YE-B6 的 G+C 含量为 55.6 mol%。基于其基因型、生理和生化特征,菌株 YE-B6 代表了 属中的一个新种,命名为 sp. nov。新种的模式菌株为 YE-B6(=MCCC 1A13131=KCTC 62716)。

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