School of Marine Sciences, Ningbo University, Ningbo, PR China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3202-3206. doi: 10.1099/ijsem.0.003610.
A Gram-stain-negative, rod-shaped and facultative anaerobic strain named YE-B6 was isolated from the intestine of abalone, . The reactions of oxidase and catalase were both positive. Strain YE-B6 could grow at 10-37 °C (optimum, 28-33 °C), at pH 7-9 (optimum, pH 7) and in salinity of 0-8 % NaCl (w/v; optimum, 2 %). The 16S rRNA gene sequence of strain YE-B6 had maximum sequence similarities to H1304 (98.0 %), DQHS21 (97.4 %), JC131 (97.4 %) and CL-GR15 (97.3 %). Results of phylogenetic analysis showed that it was affiliated to the genus within the order in the . The respiratory quinone of strain YE-B6 was Q-10. The cellular fatty acids mainly comprised C 7 and summed feature 3 (Cω6 and/or Cω7). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified aminolipids, two unidentified polar lipids and one unidentified phospholipid. The G+C content of strain YE-B6 was 55.6 mol%. Based on its genotypic, physiological and biochemical characteristics, strain YE-B6 represented a novel species within the genus , for which the name sp. nov. is proposed. The type strain of the new species is YE-B6 (=MCCC 1A13131=KCTC 62716).
一株从鲍鱼肠道中分离得到的革兰氏阴性、杆状、兼性厌氧菌株，命名为 YE-B6。氧化酶和过氧化氢酶反应均为阳性。菌株 YE-B6 可以在 10-37°C（最适温度为 28-33°C）、pH7-9（最适 pH 为 7）和 0-8%NaCl（w/v；最适 2%NaCl）的条件下生长。菌株 YE-B6 的 16S rRNA 基因序列与 H1304（98.0%）、DQHS21（97.4%）、JC131（97.4%）和 CL-GR15（97.3%）的相似度最高。系统发育分析结果表明，该菌株属于 属，在 目 科中。菌株 YE-B6 的呼吸醌为 Q-10。细胞脂肪酸主要由 C 7 和特征性脂肪酸 3（Cω6 和/或 Cω7）组成。极性脂类主要由二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰单甲乙醇胺、两种未鉴定的胺脂、两种未鉴定的极性脂类和一种未鉴定的磷脂组成。菌株 YE-B6 的 G+C 含量为 55.6 mol%。基于其基因型、生理和生化特征，菌株 YE-B6 代表了 属中的一个新种，命名为 sp. nov。新种的模式菌株为 YE-B6（=MCCC 1A13131=KCTC 62716）。
