Huang Xiao-Jun, Tang Jing-Qun, Li Man-Mei, Liu Qing, Li Yao-Lan, Fan Chun-Lin, Pei Hong, Zhao Hui-Nan, Wang Ying, Ye Wen-Cai
a Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University , Guangzhou 510632 , China.
J Asian Nat Prod Res. 2014;16(9):910-21. doi: 10.1080/10286020.2014.954554. Epub 2014 Sep 19.
A new ursane-type triterpenoid saponin, flaccidoside IV (1), and three new oleanane-type triterpenoid saponins, flaccidosides V-VII (2-4), along with 17 known saponins (5-21), were isolated from the rhizomes of Anemone flaccida. The structures of the new triterpenoid saponins were determined based on spectroscopic analyses and chemical methods. All the isolated saponins were tested for their inhibitory activities on lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophages, and several bisdesmosidic oleanane-type triterpenoid saponins (2, 7, and 10) showed significant inhibitory activities, which indicated they had potential anti-inflammatory activities under their noncytotoxic concentrations in vitro.
从威灵仙根茎中分离得到一个新的乌苏烷型三萜皂苷——萎陵菜苷IV（1）、三个新的齐墩果烷型三萜皂苷——萎陵菜苷V - VII（2 - 4），以及17个已知皂苷（5 - 21）。通过光谱分析和化学方法确定了新三萜皂苷的结构。对所有分离得到的皂苷进行了在RAW264.7巨噬细胞中抑制脂多糖诱导一氧化氮产生的活性测试，几个双糖链齐墩果烷型三萜皂苷（2、7和10）表现出显著的抑制活性，这表明它们在体外非细胞毒性浓度下具有潜在的抗炎活性。
