Statens Serum Institut, Copenhagen, Denmark.
Adv Clin Chem. 2017;81:43-96. doi: 10.1016/bs.acc.2017.01.002. Epub 2017 Feb 24.
Peptide antibodies, with their high specificities and affinities, are invaluable reagents for peptide and protein recognition in biological specimens. Depending on the application and the assay, in which the peptide antibody is to used, several factors influence successful antibody production, including peptide selection and antibody screening. Peptide antibodies have been used in clinical laboratory diagnostics with great success for decades, primarily because they can be produced to multiple targets, recognizing native wildtype proteins, denatured proteins, and newly generated epitopes. Especially mutation-specific peptide antibodies have become important as diagnostic tools in the detection of various cancers. In addition to their use as diagnostic tools in malignant and premalignant conditions, peptide antibodies are applied in all other areas of clinical laboratory diagnostics, including endocrinology, hematology, neurodegenerative diseases, cardiovascular diseases, infectious diseases, and amyloidoses.
肽抗体具有高度特异性和亲和力，是生物标本中肽和蛋白质识别的宝贵试剂。根据应用和检测方法的不同，肽抗体的成功生产受到多种因素的影响，包括肽的选择和抗体的筛选。几十年来，肽抗体已在临床实验室诊断中取得了巨大成功，主要是因为它们可以针对多个靶点产生，识别天然野生型蛋白、变性蛋白和新产生的表位。特别是突变特异性肽抗体已成为检测各种癌症的重要诊断工具。除了作为恶性和癌前状态的诊断工具外，肽抗体还应用于临床实验室诊断的所有其他领域，包括内分泌学、血液学、神经退行性疾病、心血管疾病、传染病和淀粉样变性。
