核小体DNA中未屏蔽磷酸基团的不对称侧向分布及其在DNA弯曲中的作用。
Asymmetric lateral distribution of unshielded phosphate groups in nucleosomal DNA and its role in DNA bending.
作者信息
Mirzabekov A D, Rich A
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1118-21. doi: 10.1073/pnas.76.3.1118.
Abstract
We suggest that an asymmetric charge neutralization of DNA phosphate groups produces part of the driving force for nucleosome folding. In nucleosome core particle DNA, many of the phosphate groups are neutralized by histones, and a lateral alignment of these histones along the core DNA has been demonstrated [Mirzabekov A. D., Shick, V. V., Belyavsky, A. V. & Bavykin, S. G. (1978) Proc. Natl. Acad. Sci. USA 75, 4184--4189]. Histones appear to shield DNA phosphatases asymmetrically at one side of the surface of the DNA double helix along all its length inside the core. The external side of the DNA helix remains unneutralized. The electrostatic repulsion between negatively charged unneutralized phosphates may fold the nucleosomal DNA towards the side occupied by histones.
摘要
我们认为，DNA磷酸基团的不对称电荷中和产生了核小体折叠的部分驱动力。在核小体核心颗粒DNA中，许多磷酸基团被组蛋白中和，并且已经证明这些组蛋白沿着核心DNA进行侧向排列[米尔扎别科夫A.D.，希克V.V.，别利亚夫斯基A.V.和巴夫金S.G.（1978年）美国国家科学院院刊75，4184 - 4189]。组蛋白似乎在核心内部DNA双螺旋表面的一侧沿其全长不对称地屏蔽DNA磷酸酶。DNA螺旋的外侧仍未被中和。带负电荷的未中和磷酸盐之间的静电排斥可能会使核小体DNA向组蛋白占据的一侧折叠。
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