溶液中染色质核心颗粒的结构。
The structure of the chromatin core particle in solution.
作者信息
Pardon J F, Worcester D L, Wooley J C, Cotter R I, Lilley D M, Richards R M
出版信息
Nucleic Acids Res. 1977 Sep;4(9):3199-214. doi: 10.1093/nar/4.9.3199.
Abstract
The shape and size of the nucleosomal core particle from chromatin has been examined by analysis of neutron and X-ray scattering data from dilute solutions. Calculations of scattering for many different models have been made and only one model was able to account for both the X-ray and neutron profiles. This model is an oblate structure with height about 50A and diameter 110A. The DNA is mainly confined to two annuli located at the top and bottom respectively of the core particle positioned on the outside of a compact protein core which has a height of about 40A and diameter about 73A.
摘要
通过对来自稀溶液的中子散射和X射线散射数据进行分析，研究了染色质核小体核心颗粒的形状和大小。已对许多不同模型的散射进行了计算，只有一个模型能够同时解释X射线和中子散射图谱。该模型是一个扁球体结构，高度约为50埃，直径为110埃。DNA主要局限于分别位于核心颗粒顶部和底部的两个环带，核心颗粒位于一个高度约为40埃、直径约为73埃的紧密蛋白质核心的外侧。
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