Puyang Zhen, Gong Hai-Qing, He Shi-Gang, Troy John B, Liu Xiaorong, Liang Pei-Ji
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Science, Northwestern University, Evanston, IL 60208, USA.
Exp Eye Res. 2017 Sep;162:97-103. doi: 10.1016/j.exer.2017.06.014. Epub 2017 Jun 17.
In optic neuropathies, the progressive deterioration of retinal ganglion cell (RGC) function leads to irreversible vision loss. Increasing experimental evidence suggests differing susceptibility for RGC functional subtypes. Here with multi-electrode array recordings, RGC functional loss was characterized at multiple time points in a mouse model of optic nerve crush. Firing rate, latency of response and receptive field size were analyzed for ON, OFF and ON-OFF RGCs separately. It was observed that responses and receptive fields of OFF cells were impaired earlier than ON cells after the injury. For the ON-OFF cells, the OFF component of response was also more susceptible to optic nerve injury than the ON component. Moreover, more ON transient cells survived than ON sustained cells post the crush, implying a diversified vulnerability for ON cells. Together, these data support the contention that RGCs' functional degeneration in optic nerve injury is subtype dependent, a fact that needs to be considered when developing treatments of glaucomatous retinal ganglion cell degeneration and other optic neuropathies.
在视神经病变中，视网膜神经节细胞（RGC）功能的逐渐恶化会导致不可逆的视力丧失。越来越多的实验证据表明，RGC功能亚型的易感性存在差异。在此，通过多电极阵列记录，在视神经挤压小鼠模型的多个时间点对RGC功能丧失进行了表征。分别分析了ON、OFF和ON-OFF RGC的放电率、反应潜伏期和感受野大小。观察到损伤后OFF细胞的反应和感受野比ON细胞更早受损。对于ON-OFF细胞，反应的OFF成分也比ON成分对视神经损伤更敏感。此外，挤压后存活的ON瞬态细胞比ON持续细胞更多，这意味着ON细胞存在多样化的易损性。总之，这些数据支持了这样的观点，即视神经损伤中RGC的功能退化是亚型依赖性的，这一事实在开发青光眼性视网膜神经节细胞变性和其他视神经病变的治疗方法时需要考虑。
