Beccaria Marco, Inferrera Veronica, Rigano Francesca, Gorynski Krzysztof, Purcaro Giorgia, Pawliszyn Janusz, Dugo Paola, Mondello Luigi
Chromaleont s.r.l., Messina, Italy.
Department of "Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali", University of Messina, Messina, Italy.
J Chromatogr A. 2017 Aug 4;1509:69-82. doi: 10.1016/j.chroma.2017.06.017. Epub 2017 Jun 8.
A simple, fast, and versatile method, using an ultra-high performance liquid chromatography system coupled with a low resolution (single quadrupole) mass spectrometer was optimized to perform multiclass lipid profiling of human plasma. Particular attention was made to develop a method suitable for both electrospray ionization and atmospheric pressure chemical ionization interfaces (sequentially in positive- and negative-ion mode), without any modification of the chromatographic conditions (mobile phase, flow-rate, gradient, etc.). Emphasis was given to the extrapolation of the structural information based on the fragmentation pattern obtained using atmospheric pressure chemical ionization interface, under each different ionization condition, highlighting the complementary information obtained using the electrospray ionization interface, of support for related molecule ions identification. Furthermore, mass spectra of phosphatidylserine and phosphatidylinositol obtained using the atmospheric pressure chemical ionization interface are reported and discussed for the first time.
一种简单、快速且通用的方法，使用超高效液相色谱系统与低分辨率（单四极杆）质谱仪联用，已被优化用于进行人血浆的多类脂质谱分析。特别关注开发一种适用于电喷雾电离和大气压化学电离接口（依次在正离子和负离子模式下）的方法，而无需对色谱条件（流动相、流速、梯度等）进行任何修改。重点在于基于在每种不同电离条件下使用大气压化学电离接口获得的碎片模式推断结构信息，突出使用电喷雾电离接口获得的互补信息对相关分子离子鉴定的支持。此外，首次报道并讨论了使用大气压化学电离接口获得的磷脂酰丝氨酸和磷脂酰肌醇的质谱。
