Hu Zhenyu, Wu Bingqian, Meng Fanhong, Zhou Zejian, Lu Hui, Zhao Heng
College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, People's Republic of China.
College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, People's Republic of China.
Fish Shellfish Immunol. 2017 Aug;67:554-560. doi: 10.1016/j.fsi.2017.05.066. Epub 2017 Jun 16.
Recently, molecular hydrogen has been reported to have a suppressive effect on inflammation in human and rodent models. The aim of this study was to evaluate the preventive effects of hydrogen-rich water (HRW) on zebrafish challenged by A. hydrophila. We have found an increased survival rate of bacteria-challenged zebrafish subjected to the HRW immersion treatment. Furthermore, we have revealed that HRW was able to block multiplication of A. hydrophila in zebrafish. In addition, treatment of zebrafish infected by A. hydrophila with effective concentrations of HRW strongly affected the expression of genes mediating pro-inflammatory and anti-inflammatory cytokines. There were down-regulation of selected pro-inflammatory immune response genes (IL-1β, IL-6, and NF-κB), and up-regulation of the anti-inflammatory cytokine gene (IL-10) in the spleen, kidney, and liver. This study is the first one to investigate the effects of HRW on fish infected with bacteria, and might shed new light on hydrogen's antimicrobial effects and further application in aquaculture fish species.
最近，据报道分子氢对人类和啮齿动物模型中的炎症具有抑制作用。本研究的目的是评估富氢水（HRW）对受嗜水气单胞菌攻击的斑马鱼的预防作用。我们发现，经过HRW浸泡处理的受细菌攻击的斑马鱼存活率有所提高。此外，我们还发现HRW能够阻止嗜水气单胞菌在斑马鱼体内繁殖。此外，用有效浓度的HRW处理受嗜水气单胞菌感染的斑马鱼，强烈影响了介导促炎和抗炎细胞因子的基因表达。在脾脏、肾脏和肝脏中，选定的促炎免疫反应基因（IL-1β、IL-6和NF-κB）表达下调，抗炎细胞因子基因（IL-10）表达上调。本研究是首次调查HRW对感染细菌的鱼类的影响，可能为氢的抗菌作用及其在水产养殖鱼类中的进一步应用提供新的线索。
