Carnovali Marta, Banfi Giuseppe, Mariotti Massimo
IRCCS Orthopedic Institute Galeazzi, Via C. Belgioioso 173, 20161 Milan, Italy.
School of Medicine, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, Italy.
Antioxidants (Basel). 2023 Feb 1;12(2):345. doi: 10.3390/antiox12020345.
Antioxidants represent a powerful tool for many human diseases and, in particular, molecular hydrogen has unique characteristics that make it a very promising therapeutic agent against osteoporosis. Zebrafish scales offer an innovative model in which new therapeutic approaches against secondary osteoporosis are tested. Scale bone loss obtained by prednisolone (PN) treatment is characterized by increased osteoclast activity and decreased osteoblast activity highlighted with bone enzymatic assays. We used this read-out system to test the therapeutic effects of hydrogen-rich water (HRW), an innovative antioxidant approach. HRW prevented osteoclast activation and bone loss in PN-treated fish scales, as verified by both biochemical and histochemical tartrate-resistant alkaline phosphatase assays. On the other hand, HRW treatment did not prevent PN-dependent osteoblast suppression, as measured by alkaline phosphatase activity. Moreover, HRW treatment did not facilitate the reparation of resorption lacunae induced in scales by PN. Our study highlighted a specific effect of HRW on adult osteoclast activity but not in osteoblasts, introducing an intriguing new antioxidant preventive approach against osteoporosis.
抗氧化剂是应对多种人类疾病的有力工具，特别是分子氢具有独特特性，使其成为一种非常有前景的抗骨质疏松治疗剂。斑马鱼鳞片提供了一种创新模型，可用于测试针对继发性骨质疏松症的新治疗方法。通过泼尼松龙（PN）处理获得的鳞片骨质流失的特征是破骨细胞活性增加和成骨细胞活性降低，这在骨酶测定中得到了突出体现。我们使用这个读数系统来测试富氢水（HRW）的治疗效果，这是一种创新的抗氧化方法。通过生化和组织化学抗酒石酸碱性磷酸酶测定证实，HRW可防止PN处理的鱼鳞中的破骨细胞活化和骨质流失。另一方面，通过碱性磷酸酶活性测量，HRW处理并不能防止PN依赖的成骨细胞抑制。此外，HRW处理并不能促进PN在鳞片中诱导的吸收陷窝的修复。我们的研究突出了HRW对成年破骨细胞活性的特定作用，而对成骨细胞没有作用，从而引入了一种针对骨质疏松症的有趣的新型抗氧化预防方法。
