induction method in adult zebrafish () as animal infection models.

BACKGROUND AND AIM

Zebrafish are frequently used as model organisms in scientific research as their genes mirror those of humans. bacteria can infect humans and animals, mainly fish. This study aimed to identify the concentration and route of infection in adult zebrafish. Zebrafish had been used as a challenge test by analyzing their hematological profiles, blood glucose levels, and survival rates.

MATERIALS AND METHODS

Induction of cell supernatant free (CSF) from bacteria in adult zebrafish was carried out via bath immersion (BI), intraperitoneal injection (IPI), intramuscular injection (IMI), and healthy zebrafish as a control (C). The bacterial concentrations were 10, 10, and 10 colony-forming units (CFU)/mL. At 24 h post-infection, the outcomes of infection were evaluated based on survival rates, hematological profiles, and blood glucose levels. A one-way analysis of variance with a confidence level of 95% was employed to examine the data.

RESULTS

In the BI, IPI, and IMI treatment groups, the survival rate of the fish reached a peak of 100%, 22%-100%, and 16%-63%, respectively, compared with the injection technique. In the IMI2 group, a 10 CFU/mL bacterial concentration was determined to correspond to the lethal dosage 50. All infection groups had lower erythrocyte and hemoglobin counts but higher leukocyte counts than the control group. The blood sugar levels of the healthy and infected groups were not significantly different.

CONCLUSION

The route of a infection through Intramuscular injection with a concentration of 10 CFU/mL indicated a high performance compared to other techniques. This method could be developed as a reproducible challenge test.