Smith Allie Clinton, Rice Anne, Sutton Bryan, Gabrilska Rebecca, Wessel Aimee K, Whiteley Marvin, Rumbaugh Kendra P
Department of Honors Studies, Texas Tech University, Lubbock, Texas, USA.
Department of Surgery, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Infect Immun. 2017 Aug 18;85(9). doi: 10.1128/IAI.00116-17. Print 2017 Sep.
Polymicrobial interactions are complex and can influence the course of an infection, as is the case when two or more species exhibit a synergism that produces a disease state not seen with any of the individual species alone. Cell-to-cell signaling is key to many of these interactions, but little is understood about how the host environment influences polymicrobial interactions or signaling between bacteria. Chronic wounds are typically polymicrobial, with and being the two most commonly isolated species. While readily kills , the two species can coexist for long periods together in chronic wound infections. In this study, we investigated the ability of components of the wound environment to modulate interactions between and We demonstrate that quorum sensing is inhibited by physiological levels of serum albumin, which appears to bind and sequester some homoserine lactone quorum signals, resulting in the inability of to produce virulence factors that kill These data could provide important clues regarding the virulence of in albumin-depleted versus albumin-rich infection sites and an understanding of the nature of friendly versus antagonistic interactions between and .
多种微生物间的相互作用很复杂，并且能够影响感染的进程，就像当两个或更多物种表现出协同作用从而产生单独任何一个物种都不会出现的疾病状态时那样。细胞间信号传导是许多此类相互作用的关键，但对于宿主环境如何影响多种微生物间的相互作用或细菌间的信号传导却知之甚少。慢性伤口通常是多种微生物感染，其中[具体物种1]和[具体物种2]是最常分离出的两种物种。虽然[具体物种1]能轻易杀死[具体物种2]，但在慢性伤口感染中这两种物种能长期共存。在本研究中，我们调查了伤口环境成分调节[具体物种1]和[具体物种2]之间相互作用的能力。我们证明血清白蛋白的生理水平会抑制[具体物种1]的群体感应，血清白蛋白似乎会结合并隔离一些高丝氨酸内酯群体信号，导致[具体物种1]无法产生杀死[具体物种2]的毒力因子。这些数据可能为了解在白蛋白缺乏与白蛋白丰富的感染部位[具体物种1]的毒力以及理解[具体物种1]和[具体物种2]之间友好与拮抗相互作用的本质提供重要线索。
