利用光遗传学刺激对人类癫痫模型的癫痫易感性进行研究。
Investigation of Seizure-Susceptibility in a Model of Human Epilepsy with Optogenetic Stimulation.
作者信息
Saras Arunesh, Wu Veronica V, Brawer Harlan J, Tanouye Mark A
机构信息
Department of Environmental Science, Policy and Management, Division of Organismal Biology, University of California, Berkeley, California 94720.
Department of Environmental Science, Policy and Management, Division of Organismal Biology, University of California, Berkeley, California 94720
出版信息
Genetics. 2017 Aug;206(4):1739-1746. doi: 10.1534/genetics.116.194779. Epub 2017 Jun 19.
Abstract
We examined seizure-susceptibility in a model of human epilepsy using optogenetic stimulation of (red-activatable channelrhodopsin). Photostimulation of the seizure-sensitive mutant causes behavioral paralysis that resembles paralysis caused by mechanical stimulation, in many aspects. Electrophysiology shows that photostimulation evokes abnormal seizure-like neuronal firing in followed by a quiescent period resembling synaptic failure and apparently responsible for paralysis. The pattern of neuronal activity concludes with seizure-like activity just prior to recovery. We tentatively identify the mushroom body as one apparent locus of optogenetic seizure initiation. The α/β lobes may be primarily responsible for mushroom body seizure induction.
摘要
我们使用对（红色可激活的视紫红质通道）进行光遗传学刺激的方法，在人类癫痫模型中检测了癫痫易感性。对癫痫敏感的突变体进行光刺激会导致行为性麻痹，在许多方面类似于机械刺激引起的麻痹。电生理学研究表明，光刺激在随后会引发类似于癫痫发作的异常神经元放电，随后是一段类似于突触功能衰竭的静止期，这显然是导致麻痹的原因。神经元活动模式在恢复前以类似癫痫发作的活动结束。我们初步确定蘑菇体是光遗传学癫痫发作起始的一个明显位点。α/β叶可能主要负责蘑菇体癫痫发作的诱导。
相似文献
1
Investigation of Seizure-Susceptibility in a Model of Human Epilepsy with Optogenetic Stimulation.利用光遗传学刺激对人类癫痫模型的癫痫易感性进行研究。
Genetics. 2017 Aug;206(4):1739-1746. doi: 10.1534/genetics.116.194779. Epub 2017 Jun 19.
2
Drosophila as a model for intractable epilepsy: gilgamesh suppresses seizures in para(bss1) heterozygote flies.果蝇作为耐药性癫痫模型：吉尔伽美什抑制 para(bss1)杂合子果蝇的癫痫发作。
G3 (Bethesda). 2013 Aug 7;3(8):1399-407. doi: 10.1534/g3.113.006130.
3
Drosophila sodium channel mutations: Contributions to seizure-susceptibility.果蝇钠通道突变：对癫痫易感性的影响
Exp Neurol. 2015 Dec;274(Pt A):80-7. doi: 10.1016/j.expneurol.2015.06.018. Epub 2015 Jun 18.
4
Increased persistent Na+ current contributes to seizure in the slamdance bang-sensitive Drosophila mutant.持续增强的钠离子电流导致 slamdance bang 敏感型果蝇突变体发生癫痫。
J Neurophysiol. 2011 Jul;106(1):18-29. doi: 10.1152/jn.00808.2010. Epub 2011 Mar 30.
5
Seizure control through genetic and pharmacological manipulation of Pumilio in Drosophila: a key component of neuronal homeostasis.通过对果蝇中Pumilio进行基因和药理学操纵来控制癫痫发作：神经元稳态的关键组成部分。
Dis Model Mech. 2017 Feb 1;10(2):141-150. doi: 10.1242/dmm.027045. Epub 2016 Dec 14.
6
Genetic suppression of seizure susceptibility in Drosophila.果蝇癫痫易感性的基因抑制
J Neurophysiol. 2001 Sep;86(3):1211-25. doi: 10.1152/jn.2001.86.3.1211.
7
Inappropriate Neural Activity during a Sensitive Period in Embryogenesis Results in Persistent Seizure-like Behavior.胚胎发育敏感期的不适当神经活动导致持续性癫痫样行为。
Curr Biol. 2015 Nov 16;25(22):2964-8. doi: 10.1016/j.cub.2015.09.040. Epub 2015 Nov 5.
8
Optogenetics in Drosophila Neuroscience.果蝇神经科学中的光遗传学
Methods Mol Biol. 2016;1408:167-75. doi: 10.1007/978-1-4939-3512-3_11.
9
Seizure suppression by shakB2, a gap junction mutation in Drosophila.果蝇中缝隙连接突变体shakB2对癫痫发作的抑制作用
J Neurophysiol. 2006 Feb;95(2):627-35. doi: 10.1152/jn.01059.2004. Epub 2005 Sep 28.
10
DNA topoisomerase I inhibitors ameliorate seizure-like behaviors and paralysis in a Drosophila model of epilepsy.DNA拓扑异构酶I抑制剂可改善癫痫果蝇模型中的癫痫样行为和麻痹症状。
Neuroscience. 2008 Oct 15;156(3):722-8. doi: 10.1016/j.neuroscience.2008.07.024. Epub 2008 Jul 23.
引用本文的文献
1
as a versatile model organism to study genetic epilepsies: An overview.作为研究遗传性癫痫的通用模式生物：综述。
Front Mol Neurosci. 2023 Feb 16;16:1116000. doi: 10.3389/fnmol.2023.1116000. eCollection 2023.
2
as a model for unraveling unique molecular features of epilepsy elicited by human GABA transporter 1 variants.作为一种用于揭示由人类γ-氨基丁酸转运体1变体引发的癫痫独特分子特征的模型。
Front Neurosci. 2023 Jan 19;16:1074427. doi: 10.3389/fnins.2022.1074427. eCollection 2022.
3
Epilepsy gene ensures neuropil glial ensheathment through regulating cell adhesion molecules.癫痫基因通过调节细胞粘附分子确保神经毡胶质包裹。
iScience. 2022 Dec 5;26(1):105731. doi: 10.1016/j.isci.2022.105731. eCollection 2023 Jan 20.
4
Loss-of-function variants in TIAM1 are associated with developmental delay, intellectual disability, and seizures.TIAM1 基因的功能丧失性变异与发育迟缓、智力残疾和癫痫有关。
Am J Hum Genet. 2022 Apr 7;109(4):571-586. doi: 10.1016/j.ajhg.2022.01.020. Epub 2022 Mar 2.
5
Metabolic flux from the Krebs cycle to glutamate transmission tunes a neural brake on seizure onset.从三羧酸循环到谷氨酸传递的代谢通量调节了癫痫发作起始的神经抑制作用。
PLoS Genet. 2021 Oct 29;17(10):e1009871. doi: 10.1371/journal.pgen.1009871. eCollection 2021 Oct.
6
Glia-derived temporal signals orchestrate neurogenesis in the mushroom body.胶质细胞衍生的时间信号协调蘑菇体中的神经发生。
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). doi: 10.1073/pnas.2020098118.
7
Investigating rare and ultrarare epilepsy syndromes with models.利用模型研究罕见和超罕见癫痫综合征。
Fac Rev. 2021 Jan 29;10:10. doi: 10.12703/r/10-10. eCollection 2021.
8
Anandamide Metabolites Protect against Seizures through the TRP Channel Water Witch in Drosophila melanogaster.大麻素代谢物通过果蝇中的 TRP 通道水女巫保护免受癫痫发作。
Cell Rep. 2020 Jun 2;31(9):107710. doi: 10.1016/j.celrep.2020.107710.
9
Loss of Oxidation Resistance 1, OXR1, Is Associated with an Autosomal-Recessive Neurological Disease with Cerebellar Atrophy and Lysosomal Dysfunction.氧化还原酶 1（OXR1）缺失与常染色体隐性遗传性小脑萎缩伴溶酶体功能障碍的神经退行性疾病相关。
Am J Hum Genet. 2019 Dec 5;105(6):1237-1253. doi: 10.1016/j.ajhg.2019.11.002. Epub 2019 Nov 27.
10
The Drosophila ERG channel seizure plays a role in the neuronal homeostatic stress response.果蝇 ERG 通道抽搐在神经元动态平衡应激反应中发挥作用。
PLoS Genet. 2019 Aug 8;15(8):e1008288. doi: 10.1371/journal.pgen.1008288. eCollection 2019 Aug.
本文引用的文献
1
Neural Plasticity: Dopamine Tunes the Mushroom Body Output Network.神经可塑性：多巴胺调节蘑菇体输出网络。
Curr Biol. 2016 Feb 8;26(3):R109-12. doi: 10.1016/j.cub.2015.12.023.
2
Mutations of the Calcium Channel Gene cacophony Suppress Seizures in Drosophila.钙通道基因cacophony的突变可抑制果蝇的癫痫发作。
PLoS Genet. 2016 Jan 15;12(1):e1005784. doi: 10.1371/journal.pgen.1005784. eCollection 2016 Jan.
3
Mutations in the sodium channel gene SCN2A cause neonatal epilepsy with late-onset episodic ataxia.钠通道基因SCN2A的突变会导致新生儿癫痫伴迟发性发作性共济失调。
J Neurol. 2016 Feb;263(2):334-343. doi: 10.1007/s00415-015-7984-0. Epub 2015 Dec 8.
4
Optogenetic tools for modulating and probing the epileptic network.用于调节和探测癫痫网络的光遗传学工具。
Epilepsy Res. 2015 Oct;116:15-26. doi: 10.1016/j.eplepsyres.2015.06.010. Epub 2015 Jun 21.
5
Disruption of Endocytosis with the Dynamin Mutant shibirets1 Suppresses Seizures in Drosophila.用发动蛋白突变体shibirets1破坏内吞作用可抑制果蝇的癫痫发作。
Genetics. 2015 Nov;201(3):1087-102. doi: 10.1534/genetics.115.177600. Epub 2015 Sep 4.
6
Optogenetics and epilepsy: past, present and future.光遗传学与癫痫：过去、现在和未来。
Epilepsy Curr. 2015 Jan-Feb;15(1):34-8. doi: 10.5698/1535-7597-15.1.34.
7
Modeling glial contributions to seizures and epileptogenesis: cation-chloride cotransporters in Drosophila melanogaster.模拟神经胶质细胞对癫痫发作和癫痫发生的作用：果蝇中的阳离子-氯离子共转运体
PLoS One. 2014 Jun 27;9(6):e101117. doi: 10.1371/journal.pone.0101117. eCollection 2014.
8
Stimfit: quantifying electrophysiological data with Python.Stimfit：使用 Python 量化电生理数据。
Front Neuroinform. 2014 Feb 21;8:16. doi: 10.3389/fninf.2014.00016. eCollection 2014.
9
Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship.利用红移型通道蛋白视紫红质对果蝇进行光遗传学控制，揭示了性选择对求偶行为的经验依赖性影响。
Nat Methods. 2014 Mar;11(3):325-32. doi: 10.1038/nmeth.2765. Epub 2013 Dec 22.
10
ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation.ReaChR：一种红色偏移的通道视紫红质变体，可实现深颅穿透光遗传学激发。
Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1.
Zotero 插件