Saras Arunesh, Wu Veronica V, Brawer Harlan J, Tanouye Mark A
Department of Environmental Science, Policy and Management, Division of Organismal Biology, University of California, Berkeley, California 94720.
Department of Environmental Science, Policy and Management, Division of Organismal Biology, University of California, Berkeley, California 94720
Genetics. 2017 Aug;206(4):1739-1746. doi: 10.1534/genetics.116.194779. Epub 2017 Jun 19.
We examined seizure-susceptibility in a model of human epilepsy using optogenetic stimulation of (red-activatable channelrhodopsin). Photostimulation of the seizure-sensitive mutant causes behavioral paralysis that resembles paralysis caused by mechanical stimulation, in many aspects. Electrophysiology shows that photostimulation evokes abnormal seizure-like neuronal firing in followed by a quiescent period resembling synaptic failure and apparently responsible for paralysis. The pattern of neuronal activity concludes with seizure-like activity just prior to recovery. We tentatively identify the mushroom body as one apparent locus of optogenetic seizure initiation. The α/β lobes may be primarily responsible for mushroom body seizure induction.
我们使用对（红色可激活的视紫红质通道）进行光遗传学刺激的方法，在人类癫痫模型中检测了癫痫易感性。对癫痫敏感的突变体进行光刺激会导致行为性麻痹，在许多方面类似于机械刺激引起的麻痹。电生理学研究表明，光刺激在随后会引发类似于癫痫发作的异常神经元放电，随后是一段类似于突触功能衰竭的静止期，这显然是导致麻痹的原因。神经元活动模式在恢复前以类似癫痫发作的活动结束。我们初步确定蘑菇体是光遗传学癫痫发作起始的一个明显位点。α/β叶可能主要负责蘑菇体癫痫发作的诱导。
