State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
Sci Rep. 2017 Jun 19;7(1):3827. doi: 10.1038/s41598-017-04229-z.
Gold nanomaterials have attracted considerable interest as vehicles for intracellular drug delivery. In our study, we synthesized three different shapes of methylpolyethylene glycol coated-anisotropic gold nanoparticles: stars, rods, and triangles. The cellular internalization of these nanoparticles by RAW264.7 cells was analyzed, providing a parametric evaluation of the effect of shape. The efficiency of cellular uptake of the gold nanoparticles was found to rank in the following order from lowest to highest: stars, rods, and triangles. The possible mechanisms of cellular uptake for the three types of gold nanoparticles were examined, and it was found that different shapes tended to use the various endocytosis pathways in different proportions. Our study, which has demonstrated that shape can modulate the uptake of nanoparticles into RAW264.7 cells and that triangles were the shape with the most efficient cellular uptake, provides useful guidance toward the design of nanomaterials for drug delivery.
金纳米材料作为细胞内药物递送的载体引起了广泛关注。在我们的研究中，我们合成了三种不同形状的甲基聚乙二醇包覆各向异性金纳米粒子：星型、棒型和三角形。通过 RAW264.7 细胞分析了这些纳米粒子的细胞内化，对形状的影响进行了参数评估。发现金纳米粒子的细胞摄取效率从低到高的顺序为：星型、棒型和三角形。研究了三种类型的金纳米粒子的细胞摄取的可能机制，发现不同形状往往以不同的比例利用各种内吞途径。我们的研究表明，形状可以调节 RAW264.7 细胞对纳米粒子的摄取，三角形是细胞摄取效率最高的形状，为设计用于药物递送的纳米材料提供了有用的指导。
