Wu Liping, Liu Yue, Huang Rong, Zhao Huawen, Shu Weiqun
Department of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Department of Chemistry, College of Pharmacy, Third Military Medical University, Chongqing 400038, China.
Int J Anal Chem. 2017;2017:1670812. doi: 10.1155/2017/1670812. Epub 2017 May 25.
A rapid, simple, and novel method for folate receptor (FR) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FR antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FR was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity (Δ) and the concentration of FR, with the detecting range of 0.50-37.50 ng·mL and the limit of determination of 0.05 ng·mL. The determination of FR in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation.
本文报道了一种快速、简单且新颖的叶酸受体（FR）测定方法。首先在优化条件下合成了用抗FR抗体修饰的金纳米颗粒（Au NPs）探针。当将FR加入到该探针中时，由于抗体与抗原之间的特异性相互作用，抗体修饰的Au NPs会发生聚集，导致共振瑞利散射（RRS）强度增强。RRS强度变化（Δ）与FR浓度之间存在线性关系，检测范围为0.50 - 37.50 ng·mL，测定限为0.05 ng·mL。该方法具有高选择性、高灵敏度和操作简便等优点，实现了血清样品中FR的测定。
