Li Hongchang, Cheng Yuqing, Liu Yong, Chen Bo
Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
Talanta. 2016 Sep 1;158:118-124. doi: 10.1016/j.talanta.2016.05.038. Epub 2016 May 13.
Based on the fluorescence quenching of folic acid-sensitive bovine serum albumin-directed gold nanoclusters (BSA-AuNCs) via folic acid-induced the change of environment around BSA-AuNCs, we have constructed a turn on fluorescence imaging of folate receptor overexpressed tumor cells. In this paper, the primary fluorescence intensity of BSA-AuNCs was quenched via self-assembly of folic acid onto BSA-AuNCs to produce negligible fluorescence background, the linear range of the method was 0.1-100μg/mL with the limit of detection (LOD) of 30ng/mL (S/N=3); In the presence of overexpression of folate receptor on the surface of tumor cells, the primary fluorescence intensity of BSA-AuNCs turned on by folic acid desorbing from BSA-AuNCs, the linear range of method was 0.12-2μg/mL with the LOD of 20ng/mL (S/N=3). Additionally, due to specific and high affinity of folic acid and folate receptor, the probe had high selectivity for folate receptor, other interferences hardly changed the fluorescence intensity of the probe. Moreover, the text for cytotoxicity implied that the probe had no toxicity for tumor cells. Consequently, using the fluorescence probe, satisfactory results for the turn on imaging of folate receptor overexpressed tumor cells were obtained. A novel turn-on and red fluorescent probe for folate receptor overexpressed tumor cells was developed based on the recovery of fluorescence intensity of folic acid-sensitive BSA-AuNCs.
基于叶酸敏感的牛血清白蛋白导向金纳米簇（BSA-AuNCs）通过叶酸诱导的BSA-AuNCs周围环境变化而产生的荧光猝灭，我们构建了一种用于过表达叶酸受体的肿瘤细胞的开启式荧光成像方法。在本文中，通过叶酸在BSA-AuNCs上的自组装猝灭了BSA-AuNCs的初始荧光强度，从而产生可忽略不计的荧光背景，该方法的线性范围为0.1-100μg/mL，检测限（LOD）为30ng/mL（S/N=3）；在肿瘤细胞表面存在过表达的叶酸受体时，从BSA-AuNCs上解吸的叶酸使BSA-AuNCs的初始荧光强度开启，该方法的线性范围为0.12-2μg/mL，LOD为20ng/mL（S/N=3）。此外，由于叶酸与叶酸受体具有特异性和高亲和力，该探针对叶酸受体具有高选择性，其他干扰几乎不会改变探针的荧光强度。而且，细胞毒性测试表明该探针对肿瘤细胞无毒性。因此，使用该荧光探针，对过表达叶酸受体的肿瘤细胞进行开启式成像获得了满意的结果。基于叶酸敏感的BSA-AuNCs荧光强度的恢复，开发了一种用于过表达叶酸受体的肿瘤细胞的新型开启式红色荧光探针。
