Bosch Guido, Heesen Lisa, de Melo Santos Karine, Cone John W, Pellikaan Wilbert F, Hendriks Wouter H
Animal Nutrition Group, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands.
Faculty of Veterinary Medicine, Utrecht University, PO Box 80.151, 3508 TD Utrecht, The Netherlands.
J Nutr Sci. 2017 May 24;6:e24. doi: 10.1017/jns.2017.21. eCollection 2017.
The present study aimed to evaluate the inter-individual variability in fermentation of standard fibrous substrates by faecal inocula from ten healthy adult female cats. Substrates were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from each cat. Gas production was measured continuously during the 48 h incubation and SCFA and organic matter disappearance (only SBP and WM) after incubation. Out of ten cats, nine produced faeces on the days of inoculum preparation. The substrates contrasted in terms of fermentation parameters measured. The inter-individual variability was in general lower for the more simple and pure substrates (CP, FOS, GG) than for the more complex substrates containing mixtures of fibres (SBP, WM). Furthermore, for total SCFA and gas produced, inter-individual variability was lower than for proportions of butyrate and of branched-chain fatty acids and for the parameters of gas production kinetics. It is concluded that the variability in fermentation parameters is associated with the complexity of fibrous substrates. The presented data are instrumental for the calculation of number of faecal donors required for precise characterisation of the fermentability of dietary fibres. In addition, the number of faecal donors should be adjusted to the specific fermentation parameter(s) of interest.
本研究旨在评估10只健康成年雌性猫的粪便接种物对标准纤维底物发酵的个体间变异性。底物包括柑橘果胶（CP）、低聚果糖（FOS）、瓜尔豆胶（GG）、甜菜粕（SBP）和小麦麸皮（WM）。每种底物都与每只猫的粪便接种物一起孵育。在48小时的孵育过程中持续测量气体产生量，并在孵育后测量短链脂肪酸和有机物质消失量（仅针对SBP和WM）。在10只猫中，有9只在接种物制备当天排出了粪便。所测发酵参数方面，各底物存在差异。对于更简单和纯净的底物（CP、FOS、GG），个体间变异性总体上低于含有纤维混合物的更复杂底物（SBP、WM）。此外，对于产生的总短链脂肪酸和气体，个体间变异性低于丁酸和支链脂肪酸的比例以及气体产生动力学参数。结论是，发酵参数的变异性与纤维底物的复杂性有关。所呈现的数据有助于计算精确表征膳食纤维发酵性所需的粪便供体数量。此外，粪便供体的数量应根据感兴趣的特定发酵参数进行调整。
