Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China.
Biomater Sci. 2017 Jun 27;5(7):1334-1341. doi: 10.1039/c7bm00419b.
Immobilization of membrane proteins on solid supports with high stability, favorable reusability and prevention of contamination is of great interest in nanobiology and medicine. Cell membrane coating technology enables the membrane proteins associated with their surrounding membranes to co-immobilize onto the solid matrix, largely enhancing the loading efficiency and conserving the bioactivity of the membrane proteins. Herein, we systematically illustrate the mechanism of cell membrane immobilization on porous silica beads, facilitating the fabricated biomimic carriers applied for chromatography. Rabbit red blood cell membranes were obtained via a low permeability swelling method. Batch immobilization studies were carried out to investigate the effects of the pore size of porous silica beads and incubation time on cell membrane immobilization. The absorption behavior of cell membranes could be well described by a pseudo-second-order kinetic model and the Freundlich model (a multilayer adsorption process) at 298 K, demonstrating an insertion/self-fusion mechanism involved in cell membrane coating onto the surface of porous silica beads. The insertion/self-fusion mechanism was further confirmed by confocal imaging and transmission electron microscopy.
将膜蛋白固定在固体载体上具有高稳定性、良好的可重复使用性和防止污染的特点，这在纳米生物学和医学领域具有重要意义。细胞膜涂层技术使与周围膜相关的膜蛋白能够共同固定在固体基质上，从而大大提高了负载效率并保持了膜蛋白的生物活性。本文系统地阐述了多孔硅珠上细胞膜固定的机制，促进了用于色谱的仿生载体的制备。通过低渗透性溶胀法获得兔红细胞膜。通过批处理固定化研究，考察了多孔硅珠的孔径和孵育时间对细胞膜固定化的影响。在 298 K 下，细胞膜的吸附行为可以很好地用拟二级动力学模型和 Freundlich 模型（多层吸附过程）来描述，这表明细胞膜在多孔硅珠表面的涂层涉及插入/自融合机制。插入/自融合机制通过共焦成像和透射电子显微镜进一步得到证实。
