Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Biotechnology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
J Cell Mol Med. 2017 Dec;21(12):3120-3140. doi: 10.1111/jcmm.13238. Epub 2017 Jun 19.
Long non-coding RNAs (lncRNAs) comprise a vast repertoire of RNAs playing a wide variety of crucial roles in tissue physiology in a cell-specific manner. Despite being engaged in myriads of regulatory mechanisms, many lncRNAs have still remained to be assigned any functions. A constellation of experimental techniques including single-molecule RNA in situ hybridization (sm-RNA FISH), cross-linking and immunoprecipitation (CLIP), RNA interference (RNAi), Clustered regularly interspaced short palindromic repeats (CRISPR) and so forth has been employed to shed light on lncRNA cellular localization, structure, interaction networks and functions. Here, we review these and other experimental approaches in common use for identification and characterization of lncRNAs, particularly those involved in different types of cancer, with focus on merits and demerits of each technique.
长非编码 RNA（lncRNAs）是 RNA 大家族中的一个重要成员，它们以细胞特异性的方式在组织生理学中发挥着广泛而多样的关键作用。尽管参与了无数的调控机制，但许多 lncRNAs 的功能仍有待确定。一系列的实验技术，包括单分子 RNA 原位杂交（sm-RNA FISH）、交联和免疫沉淀（CLIP）、RNA 干扰（RNAi）、成簇规律间隔短回文重复（CRISPR）等，已经被用于阐明 lncRNA 的细胞定位、结构、相互作用网络和功能。在这里，我们综述了这些和其他常用的实验方法，用于鉴定和描述 lncRNAs，特别是那些与不同类型癌症相关的 lncRNAs，重点介绍每种技术的优缺点。
