完整的微管对于细胞培养中α-微管蛋白羧基末端酪氨酸的快速周转是必需的。
Intact microtubules are required for rapid turnover of carboxyl-terminal tyrosine of alpha-tubulin in cell cultures.
作者信息
Thompson W C, Deanin G G, Gordon M W
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1318-22. doi: 10.1073/pnas.76.3.1318.
Abstract
In cultured muscle cells the carboxyl-terminal tyrosine of alpha-tubulin was shown to exchange rapidly with free tyrosine. The rapid turnover of this residue was dependent upon the presence of intact microtubules. Half-life determinations were made by two methods: (i) the cells were pulse-labeled in hypertonic medium, in which the major tyrosine incorporation was post-translational, and then chased with isotonic medium; and (ii) the cells were pulsed and chased in isotonic medium, and the post-translational component of the radioactivity of purified alpha-tubulin was calculated. Both methods yielded a half-life of 37 min or less for the terminal tyrosine residue, whereas the half-life of tubulin itself was shown to be greater than 48 hr.
摘要
在培养的肌肉细胞中，α-微管蛋白的羧基末端酪氨酸被证明能与游离酪氨酸快速交换。该残基的快速周转依赖于完整微管的存在。通过两种方法测定半衰期：（i）将细胞在高渗培养基中进行脉冲标记，其中主要的酪氨酸掺入是翻译后进行的，然后用等渗培养基进行追踪；（ii）将细胞在等渗培养基中进行脉冲和追踪，并计算纯化的α-微管蛋白放射性的翻译后成分。两种方法得出末端酪氨酸残基的半衰期均为37分钟或更短，而微管蛋白本身的半衰期则大于48小时。
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