Goldman R D
J Cell Biol. 1971 Dec;51(3):752-62. doi: 10.1083/jcb.51.3.752.
Microtubule breakdown in the presence of 5 or 40 microg/ml of colchicine is observed in BHK-21/C13 fibroblast-like cells. Several morphological and physiological effects are noted in the absence of microtubules: (a) the cells transform from fibroblast-like to epithelial-like cells; (b) the normal pattern of intracellular birefringence changes and a juxtanuclear cap of birefringent filaments is formed; (c) time-lapse cinematography demonstrates that cell locomotion is inhibited in colchicine-treated cells, even though membrane ruffling persists. The results are discussed in terms of the specific roles of microtubules in cultured cell motility and possible functional relationships of the three types of cytoplasmic fibers seen in BHK-21 cells.
在含有5或40微克/毫升秋水仙碱的情况下，在BHK - 21/C13成纤维细胞样细胞中观察到微管解聚。在没有微管的情况下，会出现几种形态学和生理学效应：(a)细胞从成纤维细胞样转变为上皮细胞样；(b)细胞内双折射的正常模式发生变化，并形成双折射细丝的近核帽；(c)延时摄影显示，秋水仙碱处理的细胞中细胞运动受到抑制，尽管膜皱褶持续存在。根据微管在培养细胞运动中的特定作用以及在BHK - 21细胞中看到的三种细胞质纤维可能的功能关系对结果进行了讨论。
