通过染色体分级分离进行基因定位：珠蛋白基因位于至少两条染色体上，三个雌激素诱导基因位于三条染色体上。

Gene localization by chromosome fractionation: globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes.

作者信息

Hughes S H, Stubblefield E, Payvar F, Engel J D, Dodgson J B, Spector D, Cordell B, Schimke R T, Varmus H E

出版信息

Proc Natl Acad Sci U S A. 1979 Mar;76(3):1348-52. doi: 10.1073/pnas.76.3.1348.

Chicken metaphase chromosomes were partially purified by rate zonal centrifugation, and DNA was prepared from each of the fractions of the sucrose gradient. The DNA was digested with various restriction enzymes and subjected to electrophoresis in agarose gels. The DNA was transferred to nitrocellulose filters (as described by Southern), and the filters were hybridized with cDNA probes. Four globin genes alpha A, alpha D, beta, and rho or epsilon are located on at least two chromosomes, and three of the estrogen-inducible genes of the hen oviduct--ovalbumin, ovomucoid, and transferrin--are on three different chromosomes. These experiments also confirm our earlier assignment of the endogenous viral sequence related to Rous-associated virus-0 to a separate (and larger) chromosome than the cellular sequence related to the transforming gene of avian sarcoma virus (cellular sarc), although it now appears that cellular sarc is on a small macrochromosome, rather than on a microchromosome.

鸡中期染色体通过速率区带离心进行部分纯化，从蔗糖梯度的每个组分中制备DNA。用各种限制性内切酶消化DNA，并在琼脂糖凝胶中进行电泳。将DNA转移到硝酸纤维素滤膜上（如Southern所述），滤膜与cDNA探针杂交。四个珠蛋白基因αA、αD、β和ρ或ε位于至少两条染色体上，母鸡输卵管的三个雌激素诱导基因——卵清蛋白、卵类粘蛋白和转铁蛋白——位于三条不同的染色体上。这些实验也证实了我们早期的研究结果，即与劳斯相关病毒0相关的内源性病毒序列位于与禽肉瘤病毒转化基因（细胞肉瘤）相关的细胞序列所在染色体之外的另一条（且更大的）染色体上，不过现在看来细胞肉瘤位于一条小的常染色体上，而非微染色体上。