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鸡胚胎α-珠蛋白基因pi'的异常结构

Unusual structure of the chicken embryonic alpha-globin gene, pi'.

作者信息

Engel J D, Rusling D J, McCune K C, Dodgson J B

出版信息

Proc Natl Acad Sci U S A. 1983 Mar;80(5):1392-6. doi: 10.1073/pnas.80.5.1392.

DOI:10.1073/pnas.80.5.1392
PMID:6572397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393603/
Abstract

We report the DNA sequence of the globin locus encoding the chicken embryonic alpha-globin, pi'. The structure differs significantly from that of the two chicken adult alpha-globin genes, alpha A and alpha D, as well as from that of previously studied adult alpha-globin genes in that the introns of the pi' gene are substantially larger than those in adult alpha-globin loci. In contrast, the pi' introns are structurally similar to the only other expressed embryonic alpha-globin gene reported to date, the human zeta gene. While completing the sequence of the pi' gene, we determined that only one chromosomal locus within the chicken genome hybridizes to a pi' central exon probe. These data lead to the conclusion that if the equimolar chicken embryonic alpha-globin polypeptides, called pi and pi', are indeed independently transcribed, then that transcription occurs from alleles of the same gene; however, we favor the possibility that the pi gene does not actually exist. This conclusion is drawn from the observation that the two chromosomal alleles of embryonic alpha-globins (represented by recombinant bacteriophage lambda CaG5 and lambda CaG7) both encode pi'.

摘要

我们报道了编码鸡胚胎α-珠蛋白pi'的珠蛋白基因座的DNA序列。其结构与两个鸡成年α-珠蛋白基因αA和αD以及先前研究的成年α-珠蛋白基因有显著差异，因为pi'基因的内含子比成年α-珠蛋白基因座中的内含子大得多。相比之下，pi'内含子在结构上与迄今为止报道的唯一另一个表达的胚胎α-珠蛋白基因——人类ζ基因相似。在完成pi'基因序列测定时，我们确定鸡基因组中只有一个染色体基因座与pi'中央外显子探针杂交。这些数据得出的结论是，如果等摩尔的鸡胚胎α-珠蛋白多肽（称为pi和pi'）确实是独立转录的，那么这种转录是从同一基因的等位基因发生的；然而，我们倾向于pi基因实际上不存在的可能性。这一结论是基于观察到胚胎α-珠蛋白的两个染色体等位基因（由重组噬菌体λCaG5和λCaG7代表）都编码pi'。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09af/393603/c7366f284e6d/pnas00631-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09af/393603/c7366f284e6d/pnas00631-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09af/393603/c7366f284e6d/pnas00631-0244-a.jpg

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本文引用的文献

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The 3' untranslated regions of the duplicated human alpha-globin genes are unexpectedly divergent.重复的人类α-珠蛋白基因的3'非翻译区出人意料地存在差异。
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