School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
J Sep Sci. 2017 Aug;40(16):3188-3195. doi: 10.1002/jssc.201700033. Epub 2017 Jul 21.
Baidianling Capsule, which is made from 16 Chinese herbs, has been widely used for treating vitiligo clinically. In this study, the sensitive and rapid method has been developed for the analysis of chemical components in Baidianling Capsule by gas chromatography-mass spectrometry in combination with retention indices and high-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry. Firstly, a total of 110 potential volatile compounds obtained from different extraction procedures including alkanes, alkenes, alkynes, ketones, ethers, aldehydes, alcohols, phenols, organic acids, esters, furans, pyrrole, acid amides, heterocycles, and oxides were detected from Baidianling Capsule by gas chromatography-mass spectrometry, of which 75 were identified by mass spectrometry in combination with the retention index. Then, a total of 124 components were tentatively identified by high-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry. Fifteen constituents from Baidianling Capsule were accurately identified by comparing the retention times with those of reference compounds, others were identified by comparing the retention times and mass spectrometry data, as well as retrieving the reference literature. This study provides a practical strategy for rapidly screening and identifying the multiple constituents of a complex traditional Chinese medicine.
白点灵胶囊由 16 种中草药制成，临床上广泛用于治疗白癜风。在这项研究中，开发了一种基于气相色谱-质谱联用保留指数和高效液相色谱-傅里叶变换离子回旋共振质谱联用的快速灵敏方法，用于分析白点灵胶囊中的化学成分。首先，通过气相色谱-质谱联用，从不同提取方法中检测到白点灵胶囊中的烷烃、烯烃、炔烃、酮、醚、醛、醇、酚、有机酸、酯、呋喃、吡咯、酰胺、杂环和氧化物等 110 种潜在挥发性化合物，其中 75 种通过质谱联用保留指数进行了鉴定。然后，通过高效液相色谱-傅里叶变换离子回旋共振质谱联用，共鉴定出 124 个成分。通过与对照品的保留时间比较，准确鉴定出白点灵胶囊中的 15 个成分，其他成分则通过保留时间和质谱数据的比较以及检索参考文献进行鉴定。本研究为快速筛选和鉴定复杂中药的多种成分提供了一种实用策略。
