School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
Shenyang Harmony Health Medical Laboratory, Shenyang, P. R. China.
J Sep Sci. 2022 Mar;45(6):1185-1194. doi: 10.1002/jssc.202100481. Epub 2022 Feb 5.
Alpiniae Oxyphyllae Fructus, as a homology of medicine and food, has been widely used in China for thousands of years. However, the existing qualitative and quantitative methods are difficult to evaluate the quality of Alpiniae Oxyphyllae Fructus samples from multiple sources. In this paper, an high-performance liquid chromatography fingerprint was established for assessing the quality of Alpiniae Oxyphyllae Fructus from different areas. Then, high-performance liquid chromatography was coupled to Fourier transform-ion cyclotron resonance mass spectrometry for characterization of the chemical compositions in Alpiniae Oxyphyllae Fructus. In fingerprint analysis, 54 common peaks were confirmed and six chromatographic peaks of them were identified. The similarity of 14 samples from different areas was between 0.990 and 1.000. Moreover, a total of 30 chemical components were characterized by high-performance liquid chromatography coupled to Fourier transform-ion cyclotron resonance mass spectrometry method, six compounds of which were decisively identified. Finally, the content of nootkatone was determined by high-performance liquid chromatography. In conclusion, the methods used in this study are efficient for qualitative and quantitative analysis of Alpiniae Oxyphyllae Fructus. Also, these methods can be used to control the quality of other traditional Chinese medicines.
益智果实，作为一种药食同源的物质，在中国已经被广泛应用了数千年。然而，现有的定性和定量方法很难评估来自多个来源的益智果实样品的质量。在本文中，建立了一种高效液相色谱指纹图谱，用于评估来自不同地区的益智果实的质量。然后，高效液相色谱法与傅里叶变换离子回旋共振质谱法相结合，用于鉴定益智果实中的化学成分。在指纹分析中，确定了 54 个共有峰，并鉴定了其中的 6 个色谱峰。来自不同地区的 14 个样品的相似度在 0.990 到 1.000 之间。此外，通过高效液相色谱-傅里叶变换离子回旋共振质谱联用技术共鉴定了 30 种化学成分，其中 6 种化合物被确证。最后，采用高效液相色谱法测定了诺卡酮的含量。综上所述，本研究中使用的方法可用于益智果实的定性和定量分析。此外，这些方法还可用于控制其他中药的质量。
