Pavlyashik G V, Zharikov A Yu, Kiselev V I
Altai State Medical University of Minzdrav of Russia, Barnaul, Russia.
Urologiia. 2017 Jun(2):24-27. doi: 10.18565/urol.2017.2.24-27.
to compare the anti-lithogenic activity of biomedical substance derived from freeze-dried porcine kidney and sodium citrate.
The experiments were conducted on Wistar rats divided into three groups of 15 animals each: control group (disease control), comparison group (sodium citrate treatment) and experimental group (treatment with biomedical substance from porcine kidneys). Experimental urolithiasis was modeled using the ethylene glycol model. On every 7th day of the 6 week experiment testing was done calcium and oxalate urine concentration and the activity of marker enzymes of renal epithelial damage: lactate dehydrogenase (LDH), -glutamyl transferase (GGT), and N-acetyl--D-glucosaminidase (NAG). At the end of the experiment, a part of the rats were decapitated and the renal tissue was tested for the oxidant status indicators of (renal thiobarbiturate reactive product content, TBRP, and total prooxidant activity, TPA) and antioxidant enzyme activities: glutathione peroxidase (GPO), superoxide dismutase (SOD) and catalase (CAT). To measure the number and size of calcium deposits formed in the renal papillary area, the Koss histochemical method was used.
The experimental findings showed developing oxalate nephrolithiasis in the control group, as indicated by urinary supersaturation of oxalate ion, increased activity of marker enzymes, oxidative stress and the formation of numerous calcium deposits in the renal papillary area. In the comparison group, the 3-week use of sodium citrate contributed to a significant decrease in nephrolithiasis: a 3 to 4-fold decrease in the activity of marker enzymes in the urine, a 3.8-fold increase in the concentration of TBRP, normalization of GPO activity; the number and size of urinary calcium deposits decreased by 3.4 and 1.9 times, respectively. In the experimental group, using biomedical substance led to an even greater therapeutic effect. LDH activity and concentration of TPRP showed 1.9 times and by 26.2% greater decrease than in the comparison group, respectively, SOD and CAT activity almost doubled, there were 3.6 times fewer calcium deposits in the field of view and their mean size was 1.7 times smaller than in the comparison group.
The study findings showed that the porcine kidney derived biomedical substance provide significantly greater antilithogenic effect than sodium citrate.
比较冻干猪肾生物医学物质与柠檬酸钠的抗结石活性。
实验在Wistar大鼠上进行，将其分为三组，每组15只动物：对照组（疾病对照）、比较组（柠檬酸钠治疗）和实验组（用猪肾生物医学物质治疗）。使用乙二醇模型建立实验性尿路结石模型。在为期6周的实验的每7天，检测尿钙和草酸浓度以及肾上皮损伤标记酶的活性：乳酸脱氢酶（LDH）、γ-谷氨酰转移酶（GGT）和N-乙酰-β-D-氨基葡萄糖苷酶（NAG）。实验结束时，部分大鼠断头，检测肾组织的氧化状态指标（肾硫代巴比妥酸反应产物含量，TBRP，和总促氧化活性，TPA）和抗氧化酶活性：谷胱甘肽过氧化物酶（GPO）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。为测量肾乳头区域形成的钙沉积物的数量和大小，采用科氏组织化学方法。
实验结果表明对照组出现了草酸钙肾结石，表现为草酸根离子尿过饱和、标记酶活性增加、氧化应激以及肾乳头区域形成大量钙沉积物。在比较组中，使用3周柠檬酸钠有助于显著降低肾结石发病率：尿中标记酶活性降低3至4倍，TBRP浓度增加3.8倍，GPO活性恢复正常；尿钙沉积物的数量和大小分别减少3.4倍和1.9倍。在实验组中，使用生物医学物质产生了更大的治疗效果。LDH活性和TPRP浓度分别比比较组降低了1.9倍和26.2%，SOD和CAT活性几乎增加了一倍，视野中的钙沉积物数量减少了3.6倍，其平均大小比比较组小1.7倍。
研究结果表明，猪肾来源的生物医学物质比柠檬酸钠具有显著更强的抗结石作用。
