Department of Urology Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, PO Box 60169, Riyadh, 11545, Saudi Arabia.
Appl Biochem Biotechnol. 2024 Aug;196(8):5419-5434. doi: 10.1007/s12010-023-04831-0. Epub 2023 Dec 30.
Urolithiasis or nephrolithiasis is a condition of kidney stone formation and is considered a painful disease of the urinary tract system. In this work, we planned to discover the therapeutic roles of myricetin on the ethylene glycol (EG)-induced nephrolithiasis in rats. The experimental rats were treated with 0.75% of EG through drinking water for 4 weeks to initiate the nephrolithiasis and subsequently treated with 25 and 50 mg/kg of myricetin. The body weight and urine volume were measured regularly. After the sacrification of rats, the samples were collected, and serum and urinary biomarkers such as creatinine, urea, Ca2 + ion, and BUN, OPN, oxalate, and citrate levels were determined using assay kits. These biomarkers, the MDA level and CAT, SOD, and GPx activities, were assessed in the kidney tissue homogenates. The IL-6, IL-1β, and TNF-α levels were also quantified using respective kits. The histopathological analysis was done on the kidney tissues. Myricetin treatment did not show major changes in the body weight and kidney weight in the EG-induced rats. The treatment with 25 and 50 mg/kg of myricetin considerably reduced the urea, creatinine, BUN, Ca2 + ion, and oxalate and increased the citrate content in serum and urine samples of EG-induced rats. Further, myricetin depleted the inflammatory cytokines and MDA levels and elevated the CAT, SOD, and GPx activities in the renal tissues. The activities of ALT, AST, ALP, GGT, and LDH were also reduced by the myricetin. Furthermore, the myricetin upheld the histoarchitecture of the kidneys. The outcomes of this investigation propose that myricetin is effective in EG-induced urolithiasis probably because of its antioxidant, anti-inflammatory, and renoprotective activities. In addition, further studies are still required to verify the precise therapeutic mechanism of myricetin.
尿石症或肾结石是一种肾结石形成的疾病，被认为是一种痛苦的泌尿系统疾病。在这项工作中，我们计划发现杨梅素对乙二醇（EG）诱导的大鼠肾结石的治疗作用。实验大鼠通过饮用水每天饮用 0.75%的 EG 4 周以引发肾结石，随后用 25 和 50mg/kg 的杨梅素进行治疗。定期测量体重和尿量。大鼠牺牲后，收集样本，使用试剂盒测定血清和尿生物标志物如肌酐、尿素、Ca2+离子和 BUN、OPN、草酸盐和柠檬酸盐水平。在肾组织匀浆中评估 MDA 水平和 CAT、SOD 和 GPx 活性等这些生物标志物。使用各自的试剂盒定量测定 IL-6、IL-1β 和 TNF-α 水平。对肾组织进行组织病理学分析。杨梅素治疗对 EG 诱导的大鼠体重和肾脏重量没有明显变化。用 25 和 50mg/kg 的杨梅素治疗可显著降低 EG 诱导的大鼠血清和尿液样本中的尿素、肌酐、BUN、Ca2+离子和草酸盐，并增加柠檬酸盐含量。此外，杨梅素可消耗炎性细胞因子和 MDA 水平，并提高肾组织中的 CAT、SOD 和 GPx 活性。杨梅素还降低了 ALT、AST、ALP、GGT 和 LDH 的活性。此外，杨梅素维持了肾脏的组织形态。这项研究的结果表明，杨梅素对 EG 诱导的尿石症有效，可能是因为其具有抗氧化、抗炎和肾保护作用。此外，还需要进一步研究来验证杨梅素的确切治疗机制。
