Human Performance Lab, Appalachian State University , North Carolina Research Campus, Kannapolis, North Carolina 28081, United States.
Metabolon , 617 Davis Drive, Suite 400, Durham, North Carolina 27713, United States.
J Proteome Res. 2017 Aug 4;16(8):2924-2935. doi: 10.1021/acs.jproteome.7b00196. Epub 2017 Jun 30.
This study evaluated the effect of ingesting a flavonoid-rich supplement (329 mg/d) on total urine phenolics and shifts in plasma metabolites in overweight/obese female adults using untargeted metabolomics procedures. Participants (N = 103, 18-65 y, BMI ≥ 25 kg/m) were randomized to flavonoid (F) or placebo (P) groups for 12 weeks with blood and 24 h urine samples collected prestudy and after 4 and 12 weeks in a parallel design. Supplements were prepared as chewable tablets and included vitamin C, wild bilberry fruit extract, green tea leaf extract, quercetin, caffeine, and omega 3 fatty acids. At 4 weeks, urine total phenolics increased 24% in F versus P with similar changes at 12 weeks (interaction effect, P = 0.041). Groups did not differ in markers of inflammation (IL-6, MCP-1, CRP) or oxidative stress (oxLDL, FRAP). Metabolomics data indicated shifts in 63 biochemicals in F versus P with 70% from the lipid and xenobiotics superpathways. The largest fold changes in F were measured for three gut-derived phenolics including 3-methoxycatechol sulfate, 3-(3-hydroxyphenyl)propanoic acid sulfate, and 1,2,3-benzenetriol sulfate (interaction effects, p ≤ 0.050). This randomized clinical trial of overweight/obese women showed that 12 weeks ingestion of a mixed flavonoid nutrient supplement was associated with a corresponding increase in urine total phenolics and gut-derived phenolic metabolites.
本研究采用非靶向代谢组学方法，评估了摄入富含类黄酮的补充剂（329 毫克/天）对超重/肥胖成年女性尿液总酚和血浆代谢物变化的影响。参与者（N=103，18-65 岁，BMI≥25kg/m）按随机分为黄酮类（F）或安慰剂（P）组，进行 12 周的平行设计，在研究前和 4 周及 12 周后采集血液和 24 小时尿液样本。补充剂制备成咀嚼片，包含维生素 C、野生越桔果提取物、绿茶提取物、槲皮素、咖啡因和欧米伽 3 脂肪酸。在 4 周时，F 组尿液总酚增加 24%，而 P 组则增加 12 周（交互效应，P=0.041）。两组在炎症标志物（IL-6、MCP-1、CRP）或氧化应激标志物（oxLDL、FRAP）上没有差异。代谢组学数据表明，F 组与 P 组相比，有 63 种生物化学物质发生变化，其中 70%来自脂质和外源性化合物途径。F 组中最大的变化倍数是三种肠道衍生的酚类物质，包括 3-甲氧基儿茶酚硫酸盐、3-（3-羟基苯基）丙酸硫酸盐和 1,2,3-苯三醇硫酸盐（交互效应，p≤0.050）。本研究对超重/肥胖女性进行的随机临床试验表明，12 周摄入混合类黄酮营养补充剂与尿液总酚和肠道衍生酚类代谢物的相应增加有关。
