Human Performance Laboratory, Appalachian State University, North Carolina Research Campus, Kannapolis, NC 28081, USA.
Food Bioprocessing and Nutrition Sciences, Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA.
Nutrients. 2018 Nov 9;10(11):1718. doi: 10.3390/nu10111718.
Using a randomized, double-blinded, placebo-controlled, parallel group design, this investigation determined if the combination of two weeks of flavonoid supplementation (329 mg/day, quercetin, anthocyanins, flavan-3-ols mixture) and a 45-minute walking bout (62.2 ± 0.9% VO (maximal oxygen consumption rate)) enhanced the translocation of gut-derived phenolics into circulation in a group of walkers ( = 77). The walkers (flavonoid, placebo groups) were randomized to either sit or walk briskly on treadmills for 45 min (thus, four groups: placebo⁻sit, placebo⁻walk, flavonoid⁻sit, flavonoid⁻walk). A comparator group of runners ( = 19) ingested a double flavonoid dose for two weeks (658 mg/day) and ran for 2.5 h (69.2 ± 1.2% VO). Four blood samples were collected (pre- and post-supplementation, immediately post- and 24 h post-exercise/rest). Of the 76 metabolites detected in this targeted analysis, 15 increased after the 2.5 h run, and when grouped were also elevated post-exercise (versus placebo⁻sit) for the placebo⁻ and flavonoid⁻walking groups ( < 0.05). A secondary analysis showed that pre-study plasma concentrations of gut-derived phenolics in the runners were 40% higher compared to walkers ( = 0.031). These data indicate that acute exercise bouts (brisk walking, intensive running) are linked to an increased translocation of gut-derived phenolics into circulation, an effect that is amplified when combined with a two-week period of increased flavonoid intake or chronic training as a runner.
采用随机、双盲、安慰剂对照、平行组设计，本研究旨在确定黄酮类化合物补充（329mg/天，槲皮素、花青素、黄烷-3-醇混合物）和 45 分钟步行（62.2±0.9%VO（最大耗氧量））相结合是否能增强肠道衍生酚类物质在一组步行者（n=77）中的循环转移。步行者（黄酮类、安慰剂组）被随机分为静坐或在跑步机上快速行走 45 分钟（因此，分为四组：安慰剂⁻静坐、安慰剂⁻行走、黄酮类⁻静坐、黄酮类⁻行走）。一组跑步者（n=19）摄入双倍黄酮类剂量两周（658mg/天）并跑步 2.5 小时（69.2±1.2%VO）。采集了 4 份血样（补充前、后、运动/休息后即刻和 24 小时后）。在这项靶向分析中检测到的 76 种代谢物中，有 15 种在 2.5 小时跑步后增加，并且当分组时，与安慰剂⁻静坐组相比，安慰剂⁻和黄酮类⁻行走组在运动后（即，与安慰剂⁻静坐相比）也升高（<0.05）。二次分析显示，与步行者相比，跑步者的血浆中肠道衍生酚类物质的基础浓度高 40%（p=0.031）。这些数据表明，急性运动（快走、剧烈跑步）与肠道衍生酚类物质向循环中的转移增加有关，当与两周的黄酮类化合物摄入增加或作为跑步者的慢性训练相结合时，这种效应会放大。
