Thongphrom Chutimon, Kim Jong-Hwa, Bora Nagamani, Kim Wonyong
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
School of Biosciences, University of Nottingham, Sutton Bonington, UK.
Int J Syst Evol Microbiol. 2017 Jun;67(6):2008-2013. doi: 10.1099/ijsem.0.001907. Epub 2017 Jun 20.
A Gram-stain positive, non-spore-forming, non-motile, facultatively anaerobic bacterial strain, designated CAU 1319T, was isolated from sea sand and the strain's taxonomic position was investigated using a polyphasic approach. Strain CAU 1319T grew optimally at 30 °C and at pH 7.5 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain CAU 1319T belongs to the genus Tessaracoccus, and is closely related to Tessaracoccus lapidicaptus IPBSL-7T (similarity 97.69 %), Tessaracoccus bendigoensis Ben 106T (similarity 95.64 %) and Tessaracoccus flavescens SST-39T (similarity 95.84 %). Strain CAU 1319T had ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-9 (H4) as the predominant menaquinone, and anteiso-C15 : 0 as the major fatty acid. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol, two unidentified aminolipids, three unidentified phospholipids and one unidentified glycolipid. Predominant polyamines were spermine and spermidine. The DNA-DNA hybridization value between strain CAU 1319T and T. lapidicaptus IPBSL-7T was 24 %±0.2. The DNA G+C content of the novel strain was 69.5 mol%. On the basis of phenotypic and chemotaxonomic properties, as well as phylogenetic relatedness, strain CAU 1319Tshould be classified as a novel species of the genus Tessaracoccus, for which the name Tessaracoccus arenae sp. nov. is proposed. The type strain is CAU 1319T(=KCTC 39760T=NBRC 111973T).
从海砂中分离出一株革兰氏染色阳性、不产芽孢、无运动性、兼性厌氧的细菌菌株，命名为CAU 1319T，并采用多相分类法研究了该菌株的分类地位。菌株CAU 1319T在30 °C、pH 7.5以及2 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株CAU 1319T属于球孢菌属，与石栖球孢菌IPBSL-7T（相似度97.69 %）、本迪戈球孢菌Ben 106T（相似度95.64 %）和微黄球孢菌SST-39T（相似度95.84 %）密切相关。菌株CAU 1319T的细胞壁肽聚糖中的诊断性二氨基酸为ll-二氨基庚二酸，主要甲基萘醌为MK-9（H4），主要脂肪酸为anteiso-C15 : 0。极性脂质由磷脂酰甘油、磷脂酰肌醇、两种未鉴定的氨基脂质、三种未鉴定的磷脂和一种未鉴定的糖脂组成。主要的多胺是精胺和亚精胺。菌株CAU 1319T与石栖球孢菌IPBSL-7T之间的DNA-DNA杂交值为24 %±0.2。该新菌株的DNA G+C含量为69.5 mol%。基于表型、化学分类学特性以及系统发育相关性，菌株CAU 1319T应被归类为球孢菌属的一个新物种，为此提出新名称沙球孢菌（Tessaracoccus arenae sp. nov.）。模式菌株为CAU 1319T（=KCTC 39760T=NBRC 111973T）。
