Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China.
School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou, 510006, China.
Curr Microbiol. 2024 Jun 16;81(8):226. doi: 10.1007/s00284-024-03766-x.
A bacterium, designated strain T21, that is non-motile, rod-shaped, and formed pale white colonies, was isolated from the sludge of a wastewater treatment plant's secondary sedimentation tank in China. Strain T21 could grow at 20-40 °C (optimum growth at 30 °C), pH 3.0-10.0 (optimum growth at pH 5.0) and in the presence of 0-8.0% (w/v) NaCl (optimum growth at 2.0%). Based on phylogenetic analysis of 16S rRNA gene sequences and genome sequences, the isolate belongs to the genus Tessaracoccus in the phylum Actinomycetota. It exhibited a close relationship with Tessaracoccus palaemonis J1M15, Tessaracoccus defluvii LNB-140, Tessaracoccus flavescens SST-39, and Tessaracoccus coleopterorum HDW20. The 16S rRNA gene sequence similarities are 99.8%, 97.9%, 97.9%, and 97.8%, respectively. The major cellular fatty acids were anteiso-C and C. The main respiratory quinone was MK-9(H). The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, glycolipid, and phospholipid. Genome annotation of strain T21 predicted the presence of 2829 genes, of which 2754 are coding proteins and 59 are RNA genes. The genomic DNA G+C content was 69.2%. Based on the results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, we propose the name Tessaracoccus lacteus sp. nov. for this novel species within the genus Tessaracoccus. The type strain is T21 (=CCTCC AB 2023031 = KCTC 49936).
一株细菌，命名为 T21 株，它是非运动性的，杆状的，形成苍白的白色菌落，是从中国一家废水处理厂的二级沉淀池的污泥中分离出来的。T21 株可以在 20-40°C（最佳生长温度为 30°C）、pH 3.0-10.0（最佳生长 pH 为 5.0）和 0-8.0%（w/v）NaCl（最佳生长 2.0%）的存在下生长。基于 16S rRNA 基因序列和基因组序列的系统发育分析，该分离株属于放线菌门的 Tessaracoccus 属。它与 Tessaracoccus palaemonis J1M15、Tessaracoccus defluvii LNB-140、Tessaracoccus flavescens SST-39 和 Tessaracoccus coleopterorum HDW20 关系密切。16S rRNA 基因序列相似性分别为 99.8%、97.9%、97.9%和 97.8%。主要的细胞脂肪酸是 anteiso-C 和 C。主要的呼吸醌是 MK-9(H)。极性脂包括磷脂酰甘油、双磷脂酰甘油、糖脂和磷脂。T21 株的基因组注释预测存在 2829 个基因，其中 2754 个是编码蛋白，59 个是 RNA 基因。基因组 DNA G+C 含量为 69.2%。基于系统发育、表型、化学分类和基因型分析的结果，我们建议将这种新的 Tessaracoccus 属内的新物种命名为 Lactobacillus lacteus sp. nov。该模式菌株为 T21（=CCTCC AB 2023031=KCTC 49936）。
