Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004588. Epub 2020 Dec 8.
A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20, isolated from the intestine of the dark diving beetle . The isolate was Gram-stain-positive, facultatively anaerobic, non-motile, coccus-shaped, and formed pale orange colonies. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus in the phylum and was closely related to SST-39, JCM 17540, and NSG39, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %. The major cellular fatty acids were C 9 and anteiso-C. The main respiratory quinone was MK-9 (H). The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 69.0 %. The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan. The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20 represents a novel species within the genus . We propose the name sp. nov. The type strain is HDW20 (=KACC 21348=KCTC 49324=JCM 33674).
采用多相分类方法对从深海潜水甲虫肠道中分离到的一株新型细菌进行了表征，命名为 HDW20 菌株。该分离株革兰氏染色阳性，兼性厌氧，不运动，呈球菌状，形成淡橙色菌落。基于 16S rRNA 基因序列和基因组序列的系统发育分析表明，该分离株属于 门的 属，与 SST-39、JCM 17540 和 NSG39 关系最为密切，16S rRNA 基因序列相似度最高为 98.5%，平均核苷酸同一性（ANI）值最高为 80.6%。主要细胞脂肪酸为 C9 和 anteiso-C。主要呼吸醌为 MK-9 (H)。主要极性脂类成分为磷脂酰甘油和双磷脂酰甘油。基因组 DNA G+C 含量为 69.0%。该分离株含有 ʟʟ-二氨基庚二酸、ʟ-丙氨酸和 ʟ-赖氨酸作为氨基酸成分，以及核糖、葡萄糖和半乳糖作为细胞壁肽聚糖的糖成分。系统发育、表型、化学分类和基因型分析的结果表明，菌株 HDW20 代表了 属内的一个新种。我们建议使用 sp. nov. 这个名称。模式菌株为 HDW20（=KACC 21348=KCTC 49324=JCM 33674）。
