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本文引用的文献

1
A simple and improved method to determine cell viability in burn-injured tissue.一种用于确定烧伤组织中细胞活力的简单且改进的方法。
J Surg Res. 2017 Jul;215:83-87. doi: 10.1016/j.jss.2017.03.064. Epub 2017 Apr 7.
2
Multispectral and Photoplethysmography Optical Imaging Techniques Identify Important Tissue Characteristics in an Animal Model of Tangential Burn Excision.多光谱和光电容积描记光学成像技术可识别切线烧伤切除动物模型中的重要组织特征。
J Burn Care Res. 2016 Jan-Feb;37(1):38-52. doi: 10.1097/BCR.0000000000000317.
3
Acute discrimination between superficial-partial and deep-partial thickness burns in a preclinical model with laser speckle imaging.在激光散斑成像的临床前模型中对浅Ⅱ度和深Ⅱ度烧伤进行急性鉴别。
Burns. 2015 Aug;41(5):1058-63. doi: 10.1016/j.burns.2014.11.018. Epub 2015 Mar 24.
4
Estimation of burn depth at burn centers in the United States: a survey.美国烧伤中心烧伤深度评估：一项调查
J Burn Care Res. 2014 Nov-Dec;35(6):491-7. doi: 10.1097/BCR.0000000000000031.
5
Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues.天狼星红苦味酸染色法：评估正常和病理组织中胶原网络的有用工具。
J Histochem Cytochem. 2014 Oct;62(10):751-8. doi: 10.1369/0022155414545787. Epub 2014 Jul 14.
6
Histological assessment of tangentially excised burn eschars.切线切除烧伤焦痂的组织学评估。
Can J Plast Surg. 2010 Fall;18(3):e33-6.
7
Improved staining method for determining the extent of thermal damage to cells.用于确定细胞热损伤程度的改良染色方法。
Lasers Surg Med. 2007 Feb;39(2):128-31. doi: 10.1002/lsm.20450.
8
A comparison of non-radioactive methods for assessing viability in ex vivo cultured cancellous bone: technical note.用于评估体外培养松质骨活力的非放射性方法比较：技术说明
Eur Cell Mater. 2006 Aug 3;12:16-25; discussion 16-25. doi: 10.22203/ecm.v012a02.
9
The progression of burn depth in experimental burns: a histological and methodological study.实验性烧伤中烧伤深度的进展：一项组织学与方法学研究。
Burns. 2004 Nov;30(7):684-90. doi: 10.1016/j.burns.2004.03.021.
10
Noncontact laser Doppler imaging in burn depth analysis of the extremities.非接触式激光多普勒成像在四肢烧伤深度分析中的应用
J Burn Care Rehabil. 2003 Jul-Aug;24(4):177-86. doi: 10.1097/01.BCR.0000075966.50533.B0.

改善烧伤深度的组织学特征。

Improving the histologic characterization of burn depth.

作者信息

Gibson Angela L F, Bennett Daniel D, Taylor Lauren J

机构信息

Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.

Department of Dermatology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.

出版信息

J Cutan Pathol. 2017 Dec;44(12):998-1004. doi: 10.1111/cup.12991. Epub 2017 Jul 24.

DOI:10.1111/cup.12991
PMID:28632906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9509661/
Abstract

BACKGROUND

Visual assessment of burn wound appearance is the standard of care to determine the depth of thermal injury but often incorrectly predicts wound healing potential. Histologic evaluation of hematoxylin and eosin (H&E) stained burn tissue is prone to subjectivity and is challenging for the novice. Lactate dehydrogenase (LDH) staining may offer a simplified and consistent technique to identify burn tissue viability.

METHODS

Thirty tissue samples were obtained from 6 patients undergoing surgical excision for clinically determined deep partial thickness or full thickness burns. Tissues were stained with H&E or LDH. Each specimen was scored by 3 individuals with varying levels of skill in histologic interpretation using a standardized checklist at 2 distinct time points.

RESULTS

Agreement within raters was highest for the expert rater and lowest for the novice; however, the LDH stained tissue method had improved agreement for an experienced burn surgeon and novice. Agreement between raters was greater for the LDH stained samples which were determined to have greater viability than the corresponding H&E section in 100% of samples scored by the expert and in 80% for the novice clinician.

CONCLUSION

LDH staining offers a more consistent measure of tissue viability that can be used by experienced and novice clinicians.

摘要

背景

通过视觉评估烧伤创面外观是确定热损伤深度的护理标准，但往往会错误地预测创面愈合潜力。苏木精和伊红（H&E）染色的烧伤组织的组织学评估容易受到主观因素影响，对新手来说具有挑战性。乳酸脱氢酶（LDH）染色可能提供一种简化且一致的技术来识别烧伤组织的活力。

方法

从6例因临床诊断为深Ⅱ度或Ⅲ度烧伤而接受手术切除的患者身上获取30个组织样本。组织用H&E或LDH染色。在2个不同时间点，由3名组织学解读技能水平不同的人员使用标准化检查表对每个标本进行评分。

结果

评分者之间的一致性在专家评分者中最高，在新手评分者中最低；然而，LDH染色组织法在经验丰富的烧伤外科医生和新手之间的一致性有所提高。对于LDH染色样本，评分者之间的一致性更高，专家评分的100%样本以及新手临床医生评分的80%样本中，LDH染色样本的活力均高于相应的H&E切片。

结论

LDH染色提供了一种更一致的组织活力测量方法，经验丰富的临床医生和新手都可以使用。