Sundheim Liv Kristin, Sporastøyl Ane Halse, Wester Torjus, Salerud Göran, Kvernebo Knut
Circulation Laboratory, Department of Cardio-thoracic Surgery, Oslo University Hospital, Ullevaal, Norway.
Medical Faculty, University of Oslo, Oslo, Norway.
Microcirculation. 2017 Oct;24(7). doi: 10.1111/micc.12389.
Superficial skin papillary capillaries with blood supply from a superficial vascular plexus and regulated by local metabolic needs supply oxygen and nutrients for epithelial cell proliferation. A deep vascular plexus regulated by autonomous nerves serves body thermoregulation. In healthy volunteers, we assessed circulatory effects of a standardized skin trauma by CAVM, DRS, and LDPM to assess the measuring depth of the three techniques and to describe the acute trauma effects on nutritive and thermoregulatory perfusion.
Volunteers (n=12) were examined at baseline and after induction of a 5.0 mm×1.0 mm incision on the forearm; 30 minutes after the trauma induction, data were collected at 0-1, 2-3 and 30 mm distances.
LDPM showed hyperemia at 2-3 mm distance (35.8±15.2 a.u.), but not at 30 mm distance (7.4±2.5 a.u.) compared to baseline (8.8±1.8 a.u.). The DRS saturation increased at 2-3 mm (71.2±4.8%), but not at 30 mm (49.8±7.9%) compared to baseline (45.8±7.4%). Capillary density and flow velocities were unaffected at all distances.
The results indicate that skin nutritive papillary capillary function can be assessed by CAVM and DRS, but not with LDPM because of its dependence of the deep plexus perfusion.
浅表皮肤乳头层毛细血管由浅表血管丛供血，并受局部代谢需求调节，为上皮细胞增殖提供氧气和营养物质。由自主神经调节的深部血管丛则负责身体的体温调节。在健康志愿者中，我们通过激光散斑对比成像（CAVM）、动态红细胞散射光谱（DRS）和激光多普勒血流成像（LDPM）评估了标准化皮肤创伤的循环效应，以评估这三种技术的测量深度，并描述急性创伤对营养性和体温调节性灌注的影响。
对12名志愿者在基线时以及在前臂诱导形成一个5.0毫米×1.0毫米的切口后进行检查；创伤诱导后30分钟，在距离切口0 - 1毫米、2 - 3毫米和30毫米处收集数据。
与基线（8.8±1.8任意单位）相比，LDPM显示在2 - 3毫米距离处出现充血（35.8±15.2任意单位），但在30毫米距离处未出现（7.4±2.5任意单位）。与基线（45.8±7.4%）相比，DRS饱和度在2 - 3毫米处增加（71.2±4.8%），但在30毫米处未增加（49.8±7.9%）。所有距离处的毛细血管密度和流速均未受影响。
结果表明，皮肤营养性乳头层毛细血管功能可通过CAVM和DRS进行评估，但不能用LDPM评估，因为它依赖于深部血管丛灌注。
