Warsaw University of Life Sciences - SGGW, Department of Biophysics, 159 Nowoursynowska St., 02-776 Warsaw, Poland.
Åbo Akademi University, Centre for Process Analytical Chemistry and Sensor Technology (ProSens), Johan Gadolin Process Chemistry Centre, Biskopsgatan 8, 20500 Åbo-Turku, Finland; AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Mickiewicza 30, 30-059 Krakow, Poland.
Bioelectrochemistry. 2017 Oct;117:65-73. doi: 10.1016/j.bioelechem.2017.06.007. Epub 2017 Jun 13.
Cystic Fibrosis (CF) is the most common fatal human genetic disease. It is caused by the defect in a single anion channel protein which affects ion and water transport across the epithelial tissue. A flat multi-electrode platform of diameter 12mm, allowing for measurement of four ions: sodium, potassium, hydrogen and chloride by exchangeable/replaceable ion-selective electrodes is described. The measurement is possible owing to the architecture of the platform which accommodates all the electrodes and inlets/outlets. The platform fits to the cup and operates in a small volume of the solution bathing the living epithelial cell layer (membrane) deposited on a porous support of the cup, which allows for effective monitoring of ion concentration changes. By applying two multi-electrode platforms, it is possible to measure the ion transmembrane fluxes. The inlet and outlet tubes in the platforms allow for on-fly change of the calibrants, ion-concentration changes and ion channel blockers. Using different ion-concentration gradients and blockers of ion-transporting molecules we show for the first time that sodium ions flow from the basolateral to apical face of the cell monolayer via a paracellular route and return also via a transcellular one, while chloride anions are transported back and forth exclusively via a transcellular route.
囊性纤维化（CF）是最常见的致命性人类遗传疾病。它是由单个阴离子通道蛋白的缺陷引起的，该蛋白影响上皮组织中的离子和水转运。本文描述了一种直径为 12mm 的平面多电极平台，该平台可通过可交换/可替换的离子选择性电极测量四种离子：钠、钾、氢和氯。由于平台的结构可以容纳所有的电极和进出口，因此可以进行测量。该平台适合杯形结构，并在浸泡在多孔支撑物上的活上皮细胞层（膜）的小体积溶液中运行，这允许有效监测离子浓度变化。通过应用两个多电极平台，可以测量离子跨膜通量。平台中的进出口管允许在飞行中改变校准剂、离子浓度变化和离子通道阻滞剂。我们首次使用不同的离子浓度梯度和离子转运分子的阻滞剂表明，钠离子通过细胞旁途径从基底外侧流向细胞单层的顶端表面，然后也通过细胞内途径返回，而氯离子仅通过细胞内途径来回运输。
