Serrano-Coll Héctor, Muñoz Mónica, Camilo Beltrán Juan, Duthie Malcolm S, Cardona-Castro Nora
Instituto Colombiano de Medicina Tropical - Universidad CES, Medellín, Colombia.
Infectious Disease Research Institute (IDRI), Seattle, USA.
Trans R Soc Trop Med Hyg. 2017 Mar 1;111(3):125-131. doi: 10.1093/trstmh/trx026.
Leprosy is a complex infectious and neurological disease caused by Mycobacterium leprae. Nerve damage is related to immunological hypersensitivity responses known as leprosy reactions (LRs). Diagnostic tools to predict LRs are not available. We hypothesized that natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) would be helpful as an indicator of LRs and neuritis.
To assess the utility of NDO-LID in indicating reactions, ELISA were used to detect specific antibodies in serum samples from 80 Colombian leprosy patients (40 with and 40 without history of LRs). Responses were detected using a range of detection reagents detecting IgG, IgM or both isotypes.
Patients with a history of LRs had an increased seropositivity rate for anti-NDO-LID antibodies compared to patients without (anti-NDO-LID protein A [p=0.02], IgG anti-NDO-LID [p=0.01] and IgM anti-NDO-LID [p=0.01]). Further analyses of patients with a history of LRs indicated that both seropositivity rate and magnitude of responses were elevated among patients with neuritis versus those without neuritis (anti-NDO-LID protein A [p=0.03], IgG anti-NDO-LID [p=0.001] and IgM anti-NDO-LID [p=0.06]).
Our data indicate that testing for serum anti-NDO-LID antibodies can be a useful screen to identify patients at risk of developing LRs and neuritis.
麻风病是一种由麻风分枝杆菌引起的复杂的传染性和神经性疾病。神经损伤与被称为麻风反应（LRs）的免疫超敏反应有关。目前尚无预测麻风反应的诊断工具。我们推测天然辛基二糖-麻风病IDRI诊断试剂（NDO-LID）可能有助于作为麻风反应和神经炎的指标。
为了评估NDO-LID在指示麻风反应方面的效用，采用酶联免疫吸附测定（ELISA）检测了80名哥伦比亚麻风病患者（40名有麻风反应病史，40名无麻风反应病史）血清样本中的特异性抗体。使用一系列检测IgG、IgM或两种同种型的检测试剂来检测反应。
与无麻风反应病史的患者相比，有麻风反应病史的患者抗NDO-LID抗体的血清阳性率更高（抗NDO-LID蛋白A [p=0.02]、抗NDO-LID IgG [p=|0.01]和抗NDO-LID IgM [p=0.01]）。对有麻风反应病史的患者的进一步分析表明，有神经炎的患者与无神经炎的患者相比，血清阳性率和反应强度均升高（抗NDO-LID蛋白A [p=0.03]、抗NDO-LID IgG [p=0.001]和抗NDO-LID IgM [p=0.06]）。
我们的数据表明，检测血清抗NDO-LID抗体可能是一种有用的筛查方法，可用于识别有发生麻风反应和神经炎风险的患者。
