Yu Sumei, Teng Chunying, Bai Xin, Liang Jinsong, Song Tao, Dong Liying, Jin Yu, Qu Juanjuan
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, P.R. China.
J Microbiol Biotechnol. 2017 Aug 28;27(8):1500-1512. doi: 10.4014/jmb.1705.05021.
In this study, the siderophore-producing characteristics and conditions of sp. PZ-1 were investigated and the enhancement of siderophores on Pb uptake and translocation in were determined. Results of single factor experiment showed that glucose, pH, and Pb(NO) could stimulate PZ-1 growth and siderophore production. The maximum siderophore production of 90.52% siderophore units was obtained by response surface methodology optimization at the glucose concentration of 21.84 g/l, pH 6.18, and Pb(NO) concentration of 245.04 μmol/l. The type of siderophore was hydroxamate and its concentration in the fermentation broth amounted to 32.24 μg/ml. Results of pot experiments indicated that the siderophores enhanced to assimilate more Pb from soil with the uptake ratio from 1.04 to 2.74, and to translocate more Pb from underground to overground with the TF values from 1.21 to 1.48. The results revealed that sp. PZ-1 could produce abundant siderophores and might be potentially used to augment the phytoextraction of Pb from soil.
本研究对菌株PZ-1产铁载体的特性和条件进行了研究，并测定了铁载体对其吸收和转运铅的促进作用。单因素实验结果表明，葡萄糖、pH值和硝酸铅可刺激PZ-1生长和铁载体产生。通过响应面法优化，在葡萄糖浓度为21.84 g/l、pH值为6.18、硝酸铅浓度为245.04 μmol/l时，铁载体产量最高，为90.52%铁载体单位。铁载体类型为异羟肟酸，其在发酵液中的浓度为32.24 μg/ml。盆栽实验结果表明，铁载体增强了其从土壤中吸收更多铅的能力，吸收比例从1.04提高到2.74，并将更多铅从地下转运到地上，转运系数从1.21提高到1.48。结果表明，菌株PZ-1可产生丰富的铁载体，可能具有增强从土壤中植物提取铅的潜力。
