Brick Mandees Bakr, Hussein Mervat H, Mowafy Amr M, Hamouda Ragaa A, Ayyad Amr M, Refaay Dina A
Department of Botany, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt.
Department of Biological Sciences, Faculty of Science, New Mansoura University, New Mansoura City, Egypt.
Microb Cell Fact. 2025 Jan 4;24(1):3. doi: 10.1186/s12934-024-02618-4.
In response to iron deficiency and other environmental stressors, cyanobacteria producing siderophores can help in ameliorating plant stress and enhancing growth physiological and biochemical processes. The objective of this work was to screen the potential of Arthrospira platensis, Pseudanabaena limnetica, Nostoc carneum, and Synechococcus mundulus for siderophore production to select the most promising isolate, then to examine the potentiality of the isolated siderophore in promoting Zea mays seedling growth in an iron-limited environment.
Data of the screening experiment illustrated that Synechococcus mundulus significantly recorded the maximum highest siderophore production (78 ± 2%) while the minimum production was recorded by Nostoc carneum (24.67 ± 0.58%). Therefore, Synechococcus mundulus was chosen for the beneficiary study and the intended agricultural application. Siderophore-type identification tests proved that Synechococcus mundulus produced hydroxamate-type. The response surface approach was successful in optimizing the conditions of siderophore production in Synechococcus mundulus with actual values for maximum biomass (387.11 mg L) and siderophore production (91.84%) higher than the predicted values. The proton nuclear magnetic resonance (H NMR) analysis data and the Fourier transformer-infrared spectrum analysis (FT-IR) signify the hydroxamate nature of Synechococcus mundulus isolated siderophore. Zea mays seedlings' growth response in the hydroponic system was significantly stimulated in response to supplementation with Synechococcus mundulus siderophore in the absence of iron compared to plants grown without iron and the positive controls. Additionally, the contents of chlorophyll a, chlorophyll b, carotenoids, total carbohydrates, and total protein were all surpassed in siderophore-treated plants, which is expected due to the increased iron content.
The results introduced in this study highlighted the significant potential of Synechococcus mundulus-derived siderophore in stimulating Zea mays physicochemical growth parameters and iron uptake. Findings of this study present novel visions of cyanobacteria producing siderophores as an ecofriendly alternative candidate to synthetic iron chelators and their role in plant stress management.
为应对缺铁和其他环境应激源，产生铁载体的蓝细菌有助于缓解植物应激并促进生长生理和生化过程。本研究的目的是筛选钝顶节旋藻、湖沼伪鱼腥藻、肉色念珠藻和蒙氏聚球藻产生铁载体的潜力，以选择最有前景的分离株，然后研究分离出的铁载体在铁限制环境中促进玉米幼苗生长的潜力。
筛选实验数据表明，蒙氏聚球藻显著记录到最高的铁载体产量（78±2%），而肉色念珠藻的产量最低（24.67±0.58%）。因此，选择蒙氏聚球藻进行受益研究和预期的农业应用。铁载体类型鉴定试验证明，蒙氏聚球藻产生异羟肟酸型铁载体。响应面法成功优化了蒙氏聚球藻中铁载体的生产条件，最大生物量（387.11 mg/L）和铁载体产量（91.84%）的实际值高于预测值。质子核磁共振（H NMR）分析数据和傅里叶变换红外光谱分析（FT-IR）表明，蒙氏聚球藻分离出的铁载体具有异羟肟酸性质。与无铁培养的植物和阳性对照相比，在无铁条件下，添加蒙氏聚球藻铁载体显著刺激了水培系统中玉米幼苗的生长反应。此外，铁载体处理的植物中叶绿素a、叶绿素b、类胡萝卜素、总碳水化合物和总蛋白的含量均超过对照，这是由于铁含量增加所致。
本研究结果突出了蒙氏聚球藻衍生铁载体在刺激玉米理化生长参数和铁吸收方面的巨大潜力。本研究结果为产生铁载体的蓝细菌作为合成铁螯合剂的生态友好替代候选物及其在植物应激管理中的作用提供了新的视角。
