Guo Yang, Zhu Sheng-Long, Wu Yi-Kuan, He Zhao, Chen Yong-Quan
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China; Synergistic Innovation Center for Food Safety and Nutrition, School of Food Science and technology, Jiangnan University, 1800 Lihu Rd, Wuxi 214122, Jiangsu, People's Republic of China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China; Synergistic Innovation Center for Food Safety and Nutrition, School of Food Science and technology, Jiangnan University, 1800 Lihu Rd, Wuxi 214122, Jiangsu, People's Republic of China; School of Medicine, Jiangnan University, Wuxi, 214122, People's Republic of China.
Nutr Res. 2017 Jun;42:43-50. doi: 10.1016/j.nutres.2017.04.008. Epub 2017 May 4.
High insulin levels in obese people are considered as a risk factor to induce breast carcinogenesis. And consumption of fish oils which mainly contain omega-3 fatty acids is associated with a reduced risk of breast cancer. However, whether omega-3 free fatty acids (FFAs) modulate insulin signaling pathway to prevent breast cancer is poorly understood. The current study tested the hypothesis that omega-3 FFAs attenuate insulin-induced breast cancer cell proliferation and regulate insulin signaling pathway. We show here that omega-3 FFAs attenuate MCF-7 cell proliferation and Akt and Erk1/2 phosphorylation levels stimulated by insulin. Knockdown Shp2 by siRNA resulted in significantly elevated omega-3 FFAs-activated Akt phosphorylation but failed to change insulin-stimulated Akt and Erk1/2 phosphorylation. And viable cell number was not affected by either downregulation of Shp2 expression or Erk1/2 inhibitor U0126 treatment. These observations indicated that omega-3 FFAs attenuate insulin-promoted breast cancer cell proliferation and insulin-activated Akt phosphorylation.
肥胖人群中高胰岛素水平被认为是诱发乳腺癌发生的一个风险因素。而食用主要含有ω-3脂肪酸的鱼油与降低乳腺癌风险相关。然而，ω-3游离脂肪酸（FFAs）是否通过调节胰岛素信号通路来预防乳腺癌，目前还知之甚少。本研究检验了ω-3 FFAs减弱胰岛素诱导的乳腺癌细胞增殖并调节胰岛素信号通路这一假说。我们在此表明，ω-3 FFAs减弱了MCF-7细胞增殖以及胰岛素刺激的Akt和Erk1/2磷酸化水平。通过小干扰RNA（siRNA）敲低Shp2导致ω-3 FFAs激活的Akt磷酸化显著升高，但未能改变胰岛素刺激的Akt和Erk1/2磷酸化。而且，Shp2表达下调或Erk-1/2抑制剂U0126处理均未影响活细胞数量。这些观察结果表明，ω-3 FFAs减弱了胰岛素促进的乳腺癌细胞增殖以及胰岛素激活的Akt磷酸化。
