Bacou Elodie, Haurogné Karine, Allard Marie, Mignot Grégoire, Bach Jean-Marie, Hervé Julie, Lieubeau Blandine
IECM, INRA, Oniris, UBL, Nantes, France.
IECM, INRA, Oniris, UBL, Nantes, France.
Dev Comp Immunol. 2017 Nov;76:169-176. doi: 10.1016/j.dci.2017.06.007. Epub 2017 Jun 17.
The cross-talk between sympatho-adreno-medullar axis and innate immunity players was mainly studied in rodents. In intensive husbandry, pigs are exposed to multiple stressors inducing repeated releases of catecholamines that bind to adrenoreceptors (AR) on target cells. Among adrenoreceptors, the β2-AR is largely expressed by immune cells including macrophages. We report herein on the effects of catecholamines, through β2-AR stimulation, on pig macrophage functions activated by LPS. β2-AR stimulation of porcine macrophages prevented the LPS-induced increase in TNFα and IL-8 secretion while increasing IL-10 secretion. In contrast, treatment with a β2-agonist had no effect on anti-microbial functions. Lastly, β2-AR stimulation of macrophages reduced the expression of genes up-regulated by LPS. Altogether, we demonstrated that β2-AR stimulation of porcine macrophages prevented polarization towards a pro-inflammatory phenotype. Since porcine macrophages are a suitable model for human macrophages, our results might be relevant to appreciate catecholamine effects on human macrophages.
交感-肾上腺髓质轴与固有免疫相关因子之间的相互作用主要是在啮齿动物中进行研究的。在集约化养殖中，猪会受到多种应激源的影响，从而导致儿茶酚胺反复释放，这些儿茶酚胺会与靶细胞上的肾上腺素能受体（AR）结合。在肾上腺素能受体中，β2-AR在包括巨噬细胞在内的免疫细胞中大量表达。我们在此报告儿茶酚胺通过β2-AR刺激对LPS激活的猪巨噬细胞功能的影响。对猪巨噬细胞进行β2-AR刺激可阻止LPS诱导的TNFα和IL-8分泌增加，同时增加IL-10分泌。相反，用β2-激动剂处理对抗菌功能没有影响。最后，对巨噬细胞进行β2-AR刺激可降低LPS上调的基因的表达。总之，我们证明了对猪巨噬细胞进行β2-AR刺激可防止其向促炎表型极化。由于猪巨噬细胞是人类巨噬细胞的合适模型，我们的结果可能有助于了解儿茶酚胺对人类巨噬细胞的影响。
