Lagos Leidy, Tandberg Julia I, Becker María Inés, Winther-Larsen Hanne C
Center of Integrative Microbial Evolution, Department of Pharmaceutical Biosciences, School of Pharmacy, Faculty of Mathematics and Natural Science, University of Oslo, Norway.
Center of Integrative Microbial Evolution, Department of Pharmaceutical Biosciences, School of Pharmacy, Faculty of Mathematics and Natural Science, University of Oslo, Norway.
Fish Shellfish Immunol. 2017 Aug;67:571-574. doi: 10.1016/j.fsi.2017.06.046. Epub 2017 Jun 19.
The development of vaccines for aquaculture has been an important milestone in providing a continuous and sustainable production. Most of the vaccines currently on the market for aquaculture include oil as adjuvant. Nevertheless, several studies reported an occurrence of side effects after their use in farmed fish. As a result, there is a need for new and improved adjuvants that can stimulate the immune system while causing as few side-effects as possible. Hemocyanins are versatile macromolecules with strong immunogenic and immunomodulatory properties. Due to these characteristics, hemocyanin from Concholepas concholepas (CCH) has been biochemically characterized and evaluated as vaccine adjuvant in mice and humans. Francisellosis is a chronic granulomatous disease, which can result in high mortality depending on the host. The disease is caused by the facultative intracellular Gram-negative bacteria Francisella noatunensis, which remains an unsolved problem for the aquaculture, as no efficient vaccines are available. The aim of the present work was to investigate the immunoregulatory properties of CCH against francisellosis in an experimental zebrafish model. When immunized with CCH, zebrafish were protected from subsequent challenge with a lethal dose of Francisella noatunensis subsp. orientalis. Subsequently the mRNA expression levels of several immune-related genes were studied, including mhcii, il12a, tnfα and ifng1-1. Taken together, the data report the immunoregulatory properties of CCH and its potential use as a vaccine adjuvant for aquaculture.
水产养殖疫苗的研发是实现持续稳定生产的一个重要里程碑。目前市场上大多数水产养殖疫苗都含有油佐剂。然而，多项研究报告称，在养殖鱼类中使用这些疫苗后会出现副作用。因此，需要新型且改良的佐剂，既能刺激免疫系统，又能尽量减少副作用。血蓝蛋白是具有强大免疫原性和免疫调节特性的多功能大分子。基于这些特性，海螺血蓝蛋白（CCH）已得到生物化学表征，并在小鼠和人体中作为疫苗佐剂进行了评估。弗朗西斯菌病是一种慢性肉芽肿性疾病，根据宿主不同，可导致高死亡率。该病由兼性细胞内革兰氏阴性菌诺氏弗朗西斯菌引起，由于没有有效的疫苗，它仍然是水产养殖中一个尚未解决的问题。本研究的目的是在实验性斑马鱼模型中研究CCH对弗朗西斯菌病的免疫调节特性。用CCH免疫后，斑马鱼能够抵御随后用致死剂量的诺氏弗朗西斯菌亚种东方亚种进行的攻击。随后研究了几个免疫相关基因的mRNA表达水平，包括mhcii、il12a、tnfα和ifng1-1。综上所述，这些数据报告了CCH的免疫调节特性及其作为水产养殖疫苗佐剂的潜在用途。
